Notes on Biology experiment - There are a whole range of enzymes that catalyse the breakdown of hydrogen peroxide, occurring in both microbial, plant and animal cells.

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Notes on Biology experiment

There are a whole range of enzymes that catalyse the breakdown of hydrogen peroxide, occurring in both microbial, plant and animal cells. According to the International Union of Biochemistry these are in group 1.11 of "peroxidase" enzymes as "acting on peroxide as acceptor". The most well-known enzyme that breaks down hydrogen peroxide is called catalase or the full name is hydrogen-peroxide: hydrogen peroxide oxidoreductase (EC number 1.11.1.6). Catalase breaks hydrogen peroxide down into oxygen and water. The reaction is 2 H2O2 (+enzyme) = O2+ 2 H2O(+enzyme)

Some other information about catalase and peroxidases:

* Catalase is a relatively famous enzyme in that it it is an enzyme described as "virtual catalytic perfection" having the highest turnover and catalytic efficiency numbers numbers measured, catalysing over 10 million reactions per second.

* In higher organisms catalase is located in organelles called peroxisomes, usually in the liver and kidneys in mammals. Here catalase plays an important role in how the body detoxifies various toxic molecules in the bloodstream, ethanol being one of the important toxic molecules! It also prevents formation of carbon dioxide microbubbles in the bloodstream.
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* Commercially catalase is used in conjunction with glucose oxidase in the production of dehydrated egg powders.

* Peroxidases catalyse these reactions usually with the use of a co-factor; most are "hemoproteins" having a heme group attached to the protein (containing an Fe atom), the same group that is part of hemoglobin that transports oxygen in the bloodstream.

* Catalase usually works in conjunction with another enzyme called superoxide dismutase that removes oxygen radicals.

The actual organic mechanism of how an enzyme works is probably beyond your course, but if you would ...

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