Chromatography of Photosynthetic Pigments
Aim: To determine the different photosynthetic pigments found in spinach and find their relative front values using paper chromatography.
By grinding the spinach leaves, its pigments were extracted in order to run a thin layer of chromatography film.
The relative front values for every photosynthetic pigment are found by dividing 'a' (distance each pigment travelled from the loading line in cm) by 'b' (distance solvent travelled from the same loading line in cm).
Pigment
Color
Carotene
Yellow
Phaeophytin
Yellow-grey
Xanthophyll
Yellow-brown
Chlorophyll a
Blue-green
Chlorophyll b
green
Calculations for the Relative Fronts of Different Pigments
Pigment
a (cm)
Aim: To determine the different photosynthetic pigments found in spinach and find their relative front values using paper chromatography.
By grinding the spinach leaves, its pigments were extracted in order to run a thin layer of chromatography film.
The relative front values for every photosynthetic pigment are found by dividing 'a' (distance each pigment travelled from the loading line in cm) by 'b' (distance solvent travelled from the same loading line in cm).
Pigment
Color
Carotene
Yellow
Phaeophytin
Yellow-grey
Xanthophyll
Yellow-brown
Chlorophyll a
Blue-green
Chlorophyll b
green
Calculations for the Relative Fronts of Different Pigments
Pigment
a (cm)