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Chromatography assignment

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Chromatography assignment Objectives: 1. To identify a unknown analgesic 2. To identify the analgesic you will be given 4 known analgesics compounds. Each of which can be separated using by Thin Layer Chromatography. 3. Using thin layer chromatography the unknown analgesic will be compared to the 4 known analgesics. Spotting of the sample 1. Prepare a microcapillary tube for each analgesic and each standard 2. Immerse the small end of the tube into the sample vial until some of the sample is drawn into the tube. 3. Very gently press the small end of the tube onto the plate about one cm from the bottom of the plate. Keep the spots small and concentrated by applying the sample 5 times and allowing the spot to dry between applications. Additional spots may be required for the ibuprofen standard in order to see its spot clearly. 4. Repeat this procedure for each of the standards available. 5. Two or more samples may be applied to each plate if they are kept one cm apart. ...read more.


* Crush the tablet using a pestle an mortar * Add 1cm� of methanol * Continue to crush the tablet with the pestle and mortar until the analgesic is dissolved in the methanol * Pure the analgesic solution into a container Ensure you use a separate pestle mortar an contain to prepare your analgesic to prevent cross contamination. Identification of an unknown analgesic * On your Thin layer Chromatography plate, draw a 1 cm line from the bottom (IN PENCIL) * Using your prepared samples and a capillary tube apply the samples along the 1cm line you must though 1. ensure all samples are equally spread apart (around 1cm) 2. ensure the spots are as small as possible 3. and apply each sample 5 times to the spot * Examine the plate under a ultraviolet light to see if you have added enough analgesic, if not add more * prepare the developing chamber using a large beaker (big enough to fit your TLC plate) ...read more.


Solvent 2 - Dichloroethaneacetic acid Analgesic Rf Value Paracetamol 2.1 6.3=0.33 Anadin 3.7 6.3=0.587 Ibuprofen 3.1 7.5=0.413 Aspirin 2.8 7.5=0.373 Unknown 2.1 6.3=0.33 The unknown analgesic Rf value matches the Rf value of the paracetamol Therefore, my results indicate that the unknown analgesic is paracetamol Conclusion My objective to complete was to conduct an experiment to discover what a unknown analgesic was using 4 known analgesics. I feel I have successfully completed this objective and identified that the unknown analgesic is paracetamol. Evaluation I found that my results were accurate, as I later did find out the unknown analgesic was infact paracetamol. So I therefore evaluate that my experiment was a complete success. I would not change my method as I feel it is accurate enough to give the correct Rf values to get an accurate result. I feel the accuracy of this experiment was very good although I do not know the actual % of inaccuracy that could arise during the experiment ...read more.

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