Physiological evidence for the Trichromatic theory is provided by Dartnall et Al (1983), who made use of microspectrophotometry – where a tiny spot of light is shone onto a single cone in the eye and the amount of light absorbed is measured at different wavelengths. They discovered there are three different types of cones that respond to three different wavelengths of light – corresponding to red, green and blue, just as predicted by the Young-Helmholtz theory.
However, this theory cannot adequately explain why people with colour-blindness who cannot see red or green can usually still see yellow. According to this theory, if there is damage to the cones for red and green, perception of yellow should also be impaired.
Trichromatic theory has been challenged by the Opponent theory of colour perception, first proposed by Hering in 1872. Hering argued that in fact we perceive 4 basic colours (not 3): red, green, blue and yellow. They work in opponent pairs of red/green and blue/yellow, and we can never perceive the opposing colours simultaneously. This is why we never perceive a colour as being ‘reddish-green’ or ‘yellowish-blue’. Unlike Trichromatic theory, Opponent theory can explain colour blindness, as damage to the red/green channel should not affect perception of yellow.
Hurvich and Jameson (1957) provided quantitative data for colour opponency, by showing that every hue can be cancelled out to white by another hue with a complementary wavelength, except for the four unique hues – blue, green, yellow and red. Svaetichin also found evidence of blue-yellow and green-red colour opponency in fish retina. However, we should be cautious in generalising these results to humans as there is good evidence that colour vision systems have evolved several separate times in fish, reptiles, birds and primates. Another study, by De Valois (1960), avoided this problem by using rhesus monkeys whose colour vision system is much more similar to humans, and found evidence of colour opponency in the lateral geniculate nucleus (LGN).
Despite the Trichromatic and Opponent Theories being apparently contradictory, in fact these studies suggest that they are compatible, within a more complex system of colour perception. The Trichromatic theory simply operates at the receptor level whilst the opponent processes theory applies to the subsequent neural level of colour vision processing.