• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

A Basic Look at the Effect of temperature on the permeability of beetroot membrane

Extracts from this document...

Introduction

A Basic Look at the Effect of temperature on the permeability of beetroot membrane Analysis The graph shows the colorimeter readings increase as the temperature increases, they increase by the most at higher temperatures. This is shown by a smooth curve. This means that the beetroot samples release more dye at higher temperatures. This is because higher temperatures cause the membrane structure to break down. The membrane structure: Membranes have two layers of molecules called phospolipids to make up their structure. Phospholipis consist of a glycerol molecule plus two molecules of fatty and a phosphate group, this looks like a head with two legs, their head is attracted to water, this means they're hydrophilic. The rest of the lipid (the fatty acid legs) is hydrophobic, which means they repel water. In aqueous solutions the phospolipids automatically arrange themselves into a double layer so that the hydrophobic legs are packed inside the membrane (away from the water) and the hydrophilic heads face outwards into the aqueous solutions. ...read more.

Middle

Enzymes are always denaturing, but at higher temperatures this occurs more rapidly. But at higher temperatures there are more collisions between the substrate and the active site so this would increase the reactivity, so a good balance must be found. The optimum temperature is about 43�C, this is called the optimum temperature. As the proteins start to break down, there is no longer a barrier to the cell The lipids in the bi-layer are slightly more stable than the proteins but 'melt' and breakdown at higher temperatures too. This in effect creates 'holes' in the membrane. Also the membrane is in part held together by the interaction of proteins and lipids with the cell's cytosceleton. These also fall apart as the temperature is raised making the membrane more permeable. This means that diffusion can take place easier. Diffusion Diffusion is the passing of molecules through a membrane, from a high concentration to a low concentration. In the experiment the beetroot dye (betalain pigments) ...read more.

Conclusion

Error Reduction/Relative Influence The Instructions should be improved by adding these points - Have everyone shake the tubes moderately for 10 seconds only at given times. - Make everyone wash the beetroot until the excess dye is completely washed off (at least for three minutes) - Everyone should vary the place in the beetroot they extract the cylinders from to be cut - The classes cylinders should all be cut by the teacher (or any other person) to ensure there is minimal variation between the thickness of the discs. Conclusion The experiment was successful as it clearly showed the effect of temperature on the beetroot membrane. It showed that the membrane becomes more permeable at higher temperatures, as was expected. This was explained by the fact that proteins denature with high temperatures and the phospholipids structure changes and becomes less stable, having devastating results on the membrane structure. Despite the limitations and errors, the results of the experiment were conclusive. (c)Hugh Howlett 2005 Written by Hugh Howlett, Cheltenham College, England. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Beetroot Experiment. The purpose of this experiment is to determine the effect of temperature ...

    4 star(s)

    Then when it comes to measuring the amount of pigment released by the use of a colorimeter the value will be different. The water is also another variable which we have controlled as regular tap water has certain impurities and a marginally lower pH than pure water which, if the

  2. Marked by a teacher

    Investigating the effect of temperature on the movement of pigment through beetroot cell membranes.

    4 star(s)

    Using the colorimeter measure the absorbance value for each temperature and record this in the table of results. 11. Plot a graph of absorbance against temperature in order to analyse and compare the results. HAZARD - HOT WATER, WEAR SAFETY GLASSES.

  1. Marked by a teacher

    The Effect of Ethanol Concentration on the Permeability of Beetroot Cell Membranes to Betalain

    3 star(s)

    with 0% ethanol solution, place in colorimeter in exact position, put the cover on top and read the dial, record result to the nearest 1%, pour the liquid back in the boiling tube and clean and dry the tube thoroughly as other solutions may affect the following result.

  2. Marked by a teacher

    AN INVESTIGATION INTO HOW TEMPERATURE AFFECTS THE PERMEABILITY MEMBRANE OF A BEETROOT.

    3 star(s)

    blocks some of the holes in the cell membrane and therefore slows down the release of betalain. From the results, I found out that low temperature don't affect the membrane of a beetroot in comparison to high temperature which result in more leakage of the betalain pigment.

  1. Marked by a teacher

    The effect of temperature on the permeability of beetroot membrane

    3 star(s)

    So more pigment will be released, as a result as the temperature increase the percentage absorbance will also increase. A2: Plan a procedure * Using suitable equipment and apparatus, list the steps to be followed (there is no need to list apparatus unless unusable items are suggested)

  2. Marked by a teacher

    An investigation to examine the effects of temperature on membrane stability in beetroot, by ...

    3 star(s)

    be best for putting beetroot in; distilled water, weak detergent or an alcohol (ethanol). * The size of the sample of beetroot. * The shape of the sample of beetroot. * The volume of liquid that will be used. * How long the beetroot will be left in the liquid for.

  1. Marked by a teacher

    Effect of different temperatures on the permeability of beetroot cell membrane.

    3 star(s)

    therefore there is a positive significant correlation between the two values. Average absorbance In the average absorbance each absorbance was increased when the temperature was increased so the rank was not changed therefore the rs was 1. This means it is over the critical value (0.79)

  2. TEMPERATURE ON BEETROOT PERMEABILITY

    Increase in temperature shows that more pigment is leaking out of the membrane, showing the membrane as getting more permeable. From 0�C to 18�C, it shows that low temperature has little effect on the beetroot's membrane, because there's a very slight decrease of light transmission (shown by observing the graph,

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work