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A Colorimetric method for the estimation of glucose (or reducing sugars) in solution.

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  • Essay length: 1385 words
  • Submitted: 14/10/2003
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AS and A Level Exchange, Transport & Reproduction

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Rikin Patel

A Colorimetric method for the estimation of glucose (or reducing sugars) in solution.

Aim: the aim of phase one of the experiments is to devise a method of measuring the concentration of glucose in a solution. Two mystery substances will be used to check if the method is correct. This will tie in with phase two of the experiment, which is to find the rate of the activity of the enzyme invertase. (The enzyme invertase catalyses the reaction: Sucrose Fructose + Glucose) i.e. the product of invertase are reducing sugars.

Information on phase 1: glucose, a reducing agent will reduce an acidified purple pink solution of potassium manganate to a colorless solution of manganese ions. The partial ionic equation is:

Mn0 + 8H + 5e Mn2 + 4H20

The time taken for the loss of colure from a standard solution of permanganate is directly related to the concentration of glucose present in solution. The results will be plotted on an assay curve. From this curve the results of the mystery substances can be measure to see if the procedure of measuring the concentration of glucose in

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