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Advantage and disadvantage of using an optical and electron microscope.

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In this essay I will be talking about all the different types of Optical and Electron Microscopes and what there uses are and what the Advantage and Disadvantage of them both are.

Electron Microscope

An electron microscope uses electrons to illuminate an object. Because electrons have a smaller wavelength than light has it can resolve much smaller structures than light can. The resolution is very good. The minimum wavelength of an optical microscope is 500nm whereas the smallest wavelength of electrons in an electron microscope is round about 0.005nm.

The microscope has many different features. It has an electron gun so that it can emit electrons onto the specimen so it can create a magnified image.

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They can exceed to great magnifications

Specimens have to be placed in a vacuum, so living things cannot be studied

They have higher resolution

They are very expensive

Optical Microscope

The optical Microscope is the most used microscope. It uses light to create its magnified images.  Some Optical Microscopes can magnify specimens by up to 2,000 times.

The compound Microscope consists of two lens systems, the OBJECTIVE and the OCULAR, put at opposite ends of  closed tube. The objective consists of several lens elements that make up an enlarged image of the examined object. The lenses are set up so that the image formed by the objective lies at focal point of the ocular. The total magnification of the microscope is determined by the focal lengths of the two lens systems.

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actually two low-powered microscopes arranged so that they converge on the specimen. These provide a three-dimensional image.

The Phase Contrast Microscope illuminates the specimen using a hollow cone of light. The cone of the light is narrow and it enters the field of view of the objective. This microscope is used for studying living tissue and used mostly for biology and medicine.



Can achieve great levels of magnification

Requires operator to assume poor posture

Can study living things (e.g. cells)

Induces eye fatigue

Lowers efficiency, speed and accuracy

High staff turnover due to health problems

Difficult to use, particularly with eyeglasses

No flexibility

Difficult to share images or communicate areas of interest

Greater magnification means higher prices.

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