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Amylase Investigation

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Biology - Amylase Investigation Planning We were asked to investigate how enzymes work in different conditions for out investigation. Enzymes, also called catalysts, are in living things and there are thousand of them. Enzymes break down food by the active site on the Enzyme forming a chemical bond with a substrate and then water attacks the substrate until it is hydrolysed (split in 2). Enzymes are very specific as only one type of food will fit in a particular Enzyme, this is called the lock and key affect. We had to find out if Amylase breaks down starch faster or slower in different situations and to alter one of its factors. I will do an experiment to see if altering the temperature of Amylase will change its rate of reaction. To change the temperature of the amylase I will put it in a ice bath to get its temperature as low as possible and put it in a hot water bath above a bunsen burner to make it get to different temperatures above room temperature. I will do this by putting 5cm� of Amylase in a test tube and I will either heat it up or cool it down to a certain temperature. Then I will add 10cm� of Starch to the Amylase and test the solution every 30 seconds until the Amylase has broken down all of the starch. ...read more.


This is because if the temperature is too high or too low then the Amylase could became denatured even though at the temperature it was meant to be at the Amylase could still gradually break down the Amylase. The time needs to be the same because the Amylase has more time to break down the Starch if you allow 40 seconds in one interval instead of 30 seconds which has been allowed in others then the reading that the Iodine gives could be wrong. This is because it has had a longer time to break down the Starch. The readings which have to be taken correctly in this experiment are the temperature and the colour of the Iodine, I will make these as correct as possible by using a good thermometer and comparing the colour of the changed Iodine to the colour of normal Iodine. I need to take about 3 sets of readings as if I only took 1 set then that set of readings could be wrong but taking 3 sets then you can see that if they are all very similar then the readings are right. Obtaining Evidence To use the equipment safely I used goggles and made sure I didn't get Iodine on me as Iodine is harmful because is had a sign on the side of the bottle. I did a practice experiment to see if my experiment would work and to get a feel for it. ...read more.


I would do 37�C because I found out that 37�C is the temperature that the body should stay at despite the temperature outside for the enzymes in the metabolism to work best. I think that I have 2 mistakes in my results because in one of my sets of results it took the 16�C 3minutes and 30seconds then in another it took 7minutes and 30seconds. Also in one set of my results the colours went clear, grey or white on a different occasion then all the other times they colour the Iodine went was either black, brown, orange or yellow. A problem with my method was judging as I could not judge the colour of the iodine that well because there was such a slight difference between the oranges and the yellows. Also I may think that one colour is orange while some else might argue that it was the same colour as the iodine. Next time I would use a colour chart so I could put that against my answers and judge the colour that way. I would also do further tests into the rate of enzyme action by doing other tests on the quickest temperatures e.g. change the pH to see if Amylase works even better in alkaline or acidic conditions. Then eventually you will find the very best and worst conditions for Amylase to work in. ...read more.

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