An experiment on the use of Immobilised Glucose Isomerase
Apparatus:
- 15g of Fructose
- 1g of Immobilised Glucose Isomerase
- 8 Clinistixs’
- 250 ml beaker
- Kettle
- Thermometer
- 8 test – tubes’
- Stirring rod
- 1 pipette
- 2 syringes
Method:
Firstly 10 cm ³ of fructose solution was placed into a test tube with a syringe. Then a clinistix was dipped into the solution for 10 seconds which it was then removed and placed against the standard strip where the colour would be observed. Then 1 gram of the immobilised glucose isomerase was added to the test tube and was incubated in at 65 °c, with occasional stirring. Then after 5 minutes a small sample was removed with a pipette after allowing the liquid to cool before testing with a clinistix. And the colour was recorded for the results. This test was repeated at every 5 minutes intervals until a maximum colour had been obtained. Then the immobilised enzyme was left to settle out and then the liquid was pipette off. Then 10cm of distilled water was added to the same test tube, where the test tube was gently mixed with a stirring rod, where then the water was removed. Then a further 10cm of fructose solution was added to the test tube and a test was taken immediately. Then again the test tube was placed into a water bath of 65 °c, where here the test was repeated two times, where the results where recorded.