• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An experiment that investigates, effect of temperature On enzyme catalyse.

Extracts from this document...


An experiment that investigates, effect of temperature On enzyme catalyse Plan: I'm going to investigate the effect of temperature on enzyme catalyse. Catalyse are biological enzymes and work best between 30 and 40 degrees. Catalyse breaks down hydrogen peroxide into water & oxygen. I can alter the experiment by these four factors which affect enzyme activity: * Temperature * PH * Concentration * Inhibitors Variables: Manipulated variable Fixed variable Dependent variable Amount of hydrogen peroxide (cm3) 3,6,9,12,15 Time-10 seconds Temperature- 35 degrees Amount of yeast ( 1 full syringe) Amount of oxygen and H20 produced I will make my investigation a fair test by controlling my variables: Concentration - I will make sure to use the same amount of yeast each time {1 full syringe}. ...read more.


complex (yeast and oxygen Hydrogen peroxide) is produced Apparatus: Before I can begin my experiment I need to gather all the apparatus I will make use of. * Measuring cylinder * 250 cm3 beakers * stopwatch * thermometer Method: * I collected all my apparatus. * Then for safety reasons I wore gloves and a lab coat and a pair of safety goggles, * I started my experiment by half filling one beaker with hydrogen peroxide and labelling it. Then got the 2nd beaker and filled half with it yeast I also labelled it. * In my yeast I added a buffer solution so they resist changes in pH. ...read more.


I also found out from my results that my predication was correct I predict that if you measured concentration (hydrogen peroxide) the rate of reaction will increased too. The more hydrogen I add to in my yeast the extra H20 I produce in 10 seconds (fixed). Evaluation: My results are reliable since they back up my predication. In my investigation I could have improved more and will next time: * I shall make sure of repeating the experiment more than 2 times * Work in pairs so I can focus on the stop - watch and the other person the cylinder. * Use I pipette for an actual perfect measurements. * Checking room temperature before I do the next amount of hydrogen peroxide each time. * Using other methods e.g. gas syringe, inverted. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    An investigation into the effect of Hydrogen Peroxide concentration on Yeast Catalyse activity

    4 star(s)

    Variables As a measure of initial reaction rate I will collect the oxygen (in ml) given off over a period of 20 seconds - this is the dependant variable. I determined 20 seconds after several preliminary tests as being the most suitable for the equipment I will use.

  2. To Investigate the effect of changing temperature on the action of the enzyme catalyse, ...

    This is clearly the case with my actual results. My prediction also states that if the temperature is increased by 10?c, the rate of enzyme activity will roughly double. This is shown to be correct by my results table and my line of best fit.

  1. Investigating and analyzing the factors affecting the rate of decomposition of hydrogen peroxide by ...

    At this stage it is said to be denatured. When the enzyme is denatured we expect it not to work at all. This might seem odd as it did work at 70�C. Well there is an explination to that. It could have been because the hydrogen paroxide was reaction with impurities within the testube and caused bubles to push the bead up.

  2. I am going to investigate how the temperature affects the catalyse enzyme on hydrogen ...

    This is shown by the graph finishing in more of a straight line and this is because the enzyme became denatured and no more oxygen was produced because an enzyme substrate complex could not be formed. There is one anomalous result for 21C.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work