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An experiment that investigates, effect of temperature On enzyme catalyse.

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Introduction

An experiment that investigates, effect of temperature On enzyme catalyse Plan: I'm going to investigate the effect of temperature on enzyme catalyse. Catalyse are biological enzymes and work best between 30 and 40 degrees. Catalyse breaks down hydrogen peroxide into water & oxygen. I can alter the experiment by these four factors which affect enzyme activity: * Temperature * PH * Concentration * Inhibitors Variables: Manipulated variable Fixed variable Dependent variable Amount of hydrogen peroxide (cm3) 3,6,9,12,15 Time-10 seconds Temperature- 35 degrees Amount of yeast ( 1 full syringe) Amount of oxygen and H20 produced I will make my investigation a fair test by controlling my variables: Concentration - I will make sure to use the same amount of yeast each time {1 full syringe}. ...read more.

Middle

complex (yeast and oxygen Hydrogen peroxide) is produced Apparatus: Before I can begin my experiment I need to gather all the apparatus I will make use of. * Measuring cylinder * 250 cm3 beakers * stopwatch * thermometer Method: * I collected all my apparatus. * Then for safety reasons I wore gloves and a lab coat and a pair of safety goggles, * I started my experiment by half filling one beaker with hydrogen peroxide and labelling it. Then got the 2nd beaker and filled half with it yeast I also labelled it. * In my yeast I added a buffer solution so they resist changes in pH. ...read more.

Conclusion

I also found out from my results that my predication was correct I predict that if you measured concentration (hydrogen peroxide) the rate of reaction will increased too. The more hydrogen I add to in my yeast the extra H20 I produce in 10 seconds (fixed). Evaluation: My results are reliable since they back up my predication. In my investigation I could have improved more and will next time: * I shall make sure of repeating the experiment more than 2 times * Work in pairs so I can focus on the stop - watch and the other person the cylinder. * Use I pipette for an actual perfect measurements. * Checking room temperature before I do the next amount of hydrogen peroxide each time. * Using other methods e.g. gas syringe, inverted. ...read more.

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