Mass/ size/ surface area – I will use the same cylinder, beaker, and syringe….etc.
Time – it will be equivalent period each time.
If variables are not controlled it would not be accurate results cause there is going to be a lot of things to measure so it wouldn’t be fair.
Predication:
I predict that the more hydrogen peroxide I add to the yeast. There will be more H20 produced because if you increase concentration would speed up the reaction {which I’m not going doing}. Also increasing the concentration {which I’m doing} would speed up the reaction also.
Active site
+
+
Enzyme substrate enzyme – substrate water
{Yeast} (Hydrogen peroxide) complex (yeast and oxygen
Hydrogen peroxide) is produced
Apparatus:
Before I can begin my experiment I need to gather all the apparatus I will make use of.
- Measuring cylinder
- 250 cm3 beakers
- stopwatch
- thermometer
Method:
- I collected all my apparatus.
- Then for safety reasons I wore gloves and a lab coat and a pair of safety goggles,
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I started my experiment by half filling one beaker with hydrogen peroxide and labelling it. Then got the 2nd beaker and filled half with it yeast I also labelled it.
- In my yeast I added a buffer solution so they resist changes in pH.
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Then I got my cylinder poured in 3cm3 of hydrogen peroxide added one full syringe of yeast and at the same time quickly started my stop- watch.
- After 10 seconds I stopped the stop watch and looked at how much H20 I produced and recoded it down on a table.
- I repeated this for 6, 9, 12, and 15. I then done the whole of the experiment again.
Analysis:
I have drawn only one table but I repeated my experiment twice and got a fairly good results. I say this because my 1st attempt and 2nd are not far for each other meaning the numbers are closely related e.g. 1st attempt 20 2nd attempt 21 this shows me there are relationship occurs though my 1st and 2nd attempt. I also found out from my results that my predication was correct I predict that if you measured concentration (hydrogen peroxide) the rate of reaction will increased too. The more hydrogen I add to in my yeast the extra H20 I produce in 10 seconds (fixed).
Evaluation:
My results are reliable since they back up my predication.
In my investigation I could have improved more and will next time:
- I shall make sure of repeating the experiment more than 2 times
- Work in pairs so I can focus on the stop – watch and the other person the cylinder.
- Use I pipette for an actual perfect measurements.
- Checking room temperature before I do the next amount of hydrogen peroxide each time.
- Using other methods e.g. gas syringe, inverted.