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An Experiment that will determine the effect Of Temperature up Rate of Reaction of Catalase And Hydrogen Peroxide.

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An Experiment that will determine the effect Of Temperature up Rate of Reaction of Catalase And Hydrogen Peroxide. Introduction: This is an experiment that will unfold the effect of temperature upon the rate of reaction of Catalase and hydrogen peroxide (H O ). From this we know that catalyse is the enzyme an H O the reactant, this will produce water (H O) and oxygen (O ). H O H O O From general knowledge I already know that enzymes increase the rate of reaction without getting used up itself. This is because enzymes lower the activation energy by providing an alternative energy pathway, therefore inevitably speeds up the rate of reaction. I also know that temperature increases the rate of reaction; this is because as temperature increases the molecules will have a higher kinetic energy resulting in more movement therefore more collision will take place and more chances of successful collision. However, this increase in the rate of reaction happens until reaching an optimum point (40celsius). If the temperature exceeds this point denaturing of the enzymes occurs, this happens when the tertiary structure of the enzyme is destroyed. Aim: To investigate the effect of temperature on the rate of an enzyme controlled reaction. Prediction: As a result of the detailed scientific knowledge and understanding mentioned above I predict as I increase the temperature the rate of reaction will also increase. This will be up to the temperature the catalyst works best (optimum). From then on the rate of reaction will decrease until it stops because of the denaturing of the enzymes third structure. ...read more.


I will make sure the temperature is dead accurate before commencing with the experiment. The water bath will to do this since its thermostatically controlled, 2. Guarantee the strict amount of substrate is used each time. 3. I will also use the same potato; keep the size and surface area of the potato the same. A knife and a cork borer will assist with this. Health and Safety Risk: Goggles and Lab coats must be worn at all times to prevent solutions from contacting the eye or the skin. However, if the solutions make contact with eyes rinse thoroughly, if irritating continues alert teacher. If solutions make contact with skin wash off immediately if irritating continues alert teacher. In addition, if there is any spillage clean and dry the area effectively. Hydrogen Peroxide should be kept away from heat since it is an oxidant. Moreover, I will be using a sharp knife to cut potato which requires to be kept away from the experimental area if it is not in use. My tables of results: At 0�C Replicates: Volume of 0xygen collected in cm� Time (secs) 1 2 3 Mean 30 0.00 0.00 0.00 0.00 60 0.00 0.00 0.00 0.00 90 0.00 0.25 0.00 0.08 120 0.00 0.25 0.25 0.16 150 0.00 0.25 0.25 0.16 180 0.00 0.25 0.25 0.16 At 24�C Replicates: Volume of 0xygen collected in cm� Time (secs) 1 2 3 Mean 30 0.25 0.50 0.50 0.42 60 0.50 1.00 0.75 0.75 90 0.75 1.75 1.25 1.25 120 1.50 2.50 1.75 1.92 150 2.25 3.00 2.00 2.42 180 2.75 3.75 2.50 3.50 At 37�C Replicates: Volume of 0xygen collected in cm� Time (secs) ...read more.


This mediocre judgement reduced the reliability of my results for this specific result because the room temperature is not always 20�. * The usage of more then 1 potato also hindered the reliability of my result as different potatoes have diverse level of concentration mainly depending on the environment it thrived in and depending how fresh it is or not. * My biggest source of error occurred because it took too long for the thermostatically controlled water bath to heat up. This issue collided with the fact that I never had enough time to carry out the experiment. As a clear result of this dilemma I started some recordings of different temperature at a 1� or maximum 2�. Improvements: In the short or long term future if I was to do this experiment again there would be slight improvements made to overcome obstacles that hindered my results. For example; 1. Firstly as an alternative I would use a thermostatically controlled water bath for 20� to represent room temperature instead of assuming room temperature is 20�. 2. I will also ensure the same person reads the volume of oxygen produced from the measuring cylinder because different people might slightly read the measurements differently. 3. If I also repeated the experiment more often then my results would have been more consistent which would have further increased the reliability of my results. 4. Most importantly I would have allocated more time for the experiment so that I could have ensured that experiment was not rushed and that the thermostatically controlled water bath was at the correct temperature instead of being 1degree or 2 off. ?? ?? ?? ?? Sadiq Ali ...read more.

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