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An experiment to find out the effect of different sucrose solutions on the growth of yeast.

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Introduction

An experiment to find out the effect of different sucrose solutions on the growth of yeast. In this experiment I will be trying to find out how different amounts of sucrose solutions will effect the growth of yeast. Variables i) Temperature - If the Temperature were to increase it would have a great impact on the experiment, as it will be left for a few days (see later on in procedures). The temperatures inside the chronicle flask will all have to be at a fairly constant level, to allow the yeast cells growth effectively and at a steady rate, as this will be a fair test.

Middle

To find out whether yeast grows better in a high or low concentration of yeast I will require six test tubes. Each test tube will be filled with a different concentration of the 10% sucrose solution; the volume of the solution in each of the test tubes will be 200ml Each tube will be filled with a different concentration (as shown in Fig 1). Fig 1 Fig 2 - A table showing the content of each of the test tubes Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 How much of the solution is sucrose 4/4 3/4 1/2 1/4 1/8 Amount of sucrose (ml) 200 150 100 50 25 Amount of water (ml)

Conclusion

When counting the yeast cells make sure that the cells that are on the line are not counted more that once. To avoid this you could: if they are touching lines only count the ones on the middle of the two sides, if they touch any other two lines ignore them. This experiment will last for two days and every three hours take samples from each of the chronicle flasks, place on the haemocytometer and record the number of yeast cells there are. It will be impractical to measure it over night so there will be a difference in my results during this time, as I will be measuring the experiment from 4o'clock on the previous day till 9o'clock the next day.

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