An experiment to find out the effect of different sucrose solutions on the growth of yeast.

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An experiment to find out the effect of different sucrose solutions on the growth of yeast.

In this experiment I will be trying to find out how different amounts of sucrose solutions will effect the growth of yeast.

Variables

  1. Temperature - If the Temperature were to increase it would have a great impact on the experiment, as it will be left for a few days (see later on in procedures). The temperatures inside the chronicle flask will all have to be at a fairly constant level, to allow the yeast cells growth effectively and at a steady rate, as this will be a fair test.
  2. Volume of the solution – The volume of solution in each of the test tubes and chronicle flasks must be all the same as other wise the experiment would not be a fair test.
  3. The amount of yeast – The amount of yeast put into each of the chronicle flasks must be the same as it would not be a fair test.
  4. The sucrose concentration – It must be a known concentration varying from a high concentration to a low concentration of sucrose between the six test tubes, so it is able to see in which concentration the yeast grows best in.
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Apparatus.

  1. 6 test tubes
  2. Haemocytometer
  3. Test tube rack
  4. 6 chronicle flasks
  5. 10 % Sucrose solution
  6. Stock yeast suspension

Procedures.

To find out whether yeast grows better in a high or low concentration of yeast I will require six test tubes. Each test tube will be filled with a different concentration of the 10% sucrose solution; the volume of the solution in each of the test tubes will be 200ml

 

 

Each tube will be filled with a different concentration (as shown in Fig 1).

Fig 1

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