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An experiment to investigate how temperature affects the permeability of beetroot cell membranes

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Introduction

An experiment to investigate how temperature affects the Permeability of beetroot cell membranes Interpretation of Results: 0�C 20�C 30�C 40�C 55�C 0.24 0.28 0.75 0.79 0.22 0.26 0.35 0.41 0.81 From 0�C to 30�C there is a gradual increase of absorbency which shows that as the temperature increases it is denaturing the cell wall and cell membrane and allowing the beetroot pigment to leek out into the distilled water. As soon as the sample of beetroot is placed into the distilled water diffusion occurs naturally, which is the net movement of molecules from a region of high concentration (beetroot sample) to a region of low concentration (distilled water). From 40�C upwards there is a sudden increase of absorbency, this is because the optimum temperature of which enzymes and cells can work at is 37�C and as soon as you go above this temperature it will cause the enzymes and cells to denature and cease from working as effectively. ...read more.

Middle

The anomaly is taken from my second row of results (highlighted in red). At the temperature 40�C, the first result I collected from this temperature was 0.75 arbitrary units and from the re-test results at the same temperature I got 0.41 arbitrary units. This is a difference of 0.34 arbitrary units. This piece of data is not what I would have expected due to the first set of results, although the data at 55�C on the first and second tests are relatively the same. Evaluation of Practical Work: The anomaly which I have identified at 40�C is 0.41 arbitrary units this could be due to the size of the sample of beetroot used, if the sample was smaller than the other samples it was have less pigment which would suggested why the result is lower than expected. Another suggestion for why the result is lower than expected could be due to the loss of pigment in the preparation process, or from where the sample was taken from on the beetroot. ...read more.

Conclusion

Another inaccuracy which could have occurred was the size of the beetroot cubes, this would be due to the inaccuracy of the measuring equipment; this could explain the abnormality found within my results. If the cube was cut slightly smaller it would have reduced the surface area therefore decreasing the amount of pigment leaked from the sample. Another reason for the anomaly appearing could be due to the preparation of the sample. Many of the cells could have been damaged causing pigment to leak out so reducing the amount of pigment leaked during the experiment. A way of overcoming the inaccuracy of the measuring equipment is to have rulers which are graded more accurately which may help prevent inaccuracies occurring in the future. The experiment should be repeated more than two or three times by doing so; it would be easier to work out a clear average between the results, see any trends and you would be able to see any anomalies more clearly. ...read more.

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