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An Experiment To Investigate The Effect Of Hydrogen Peroxide (Substance) Upon Yeast (Catalase)

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Introduction

Matthew Chamberlain Brownhillls Community School An ExperimenTo Investigate The EffectOf Hydrogen Peroxide (Substance) Upon Yeast (Catalase) Background : Catalase is a protein molecule, thats reactions it catalyses are crucial to life. Catalyses converts Hydrogen Peroxide to water and oxygen. Catalase also uses Hydrogen Peroxide to oxidise toxins including Phenols, Formic Acid, Formaldehyde and Alcohols. Catalase is an enzyme found in food such as potato, liver and yeast!. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by -product of metabolism. The enzyme catalase speeds up the decomposition of hydrogen peroxide (2H O ). Hydrogen peroxide breaks down into water and oxygen. Equation : Catalse Hydrogen Peroxide Water + Oxygen Catalse 2H O 2H O + O It is able to speed up the decomposition of Hydrogen Peroxide because the shape of it matches the shape of the Hydrogen Peroxide. This type of is called an anabolic reaction as the Hydrogen Peroxide molecule is broken up into smaller peices.

Middle

This has also been shown to be true of reaction catalysed by enzymes when other conditions, such as concentration of enzyme and substrate, are constant but it is important to remember that this will only occur between approximately 4 degrees and 50 degrees Celsius. Prediction : I believe that the rate of production of O and H O will increase, with substrate concentration. This rate is most probably proportional, until the saturation point is reached. After the saturation point has been reached I believe that adding extra substrate will make no difference. I also think that the more substrate that is being used, the more enzyme will be used, so therefore the amount of O that will be given off will be higher. The rate will steadily increase when more substrate is added, this is because there will be more molecules of the enzyme that are being used which will therefore result in more reactions so the amount of oxygen is produced more rapidly.

Conclusion

6. Repeat the experiment, exactly the same, but using hydrogen peroxide concentrations of 80%, 50%, 40% and 20%. 7..As with the 100% concentration, repeat all the tests at three more times so that an average can be obtained. Repeating the experiments several times will help to produce better and more accurate results. Results: Time Taken Grams Of Yeast % Of Concentration (2H O ) Amount of 2H O (ml) 1st 2nd 3rd 4th Averages 0.2 g 100 4 17.91 19.05 18.91 17.32 18.29 0.2 g 80 4 1min33 1min47 1min36 1min44 1min40 0.2 g 50 4 3min22 3min49 3min32 4min01 3min53 0.2 g 40 4 4min19 4min31 4min27 4min41 4min29 0.2 g 20 4 6min32 5min46 5min50 6min03 6min22 (Results table) The times above are in minutes/seconds. The average times that I have worked out for each concentration are showed in the far right-hand column, I have worked them out to two decimal place. From the results I have found above I was able to plot various graphs of the rate of reaction against the concentration of Hydrogen Peroxide.

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