• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An Experiment To Investigate The Effect Of Hydrogen Peroxide (Substance) Upon Yeast (Catalase)

Extracts from this document...

Introduction

Matthew Chamberlain Brownhillls Community School An ExperimenTo Investigate The EffectOf Hydrogen Peroxide (Substance) Upon Yeast (Catalase) Background : Catalase is a protein molecule, thats reactions it catalyses are crucial to life. Catalyses converts Hydrogen Peroxide to water and oxygen. Catalase also uses Hydrogen Peroxide to oxidise toxins including Phenols, Formic Acid, Formaldehyde and Alcohols. Catalase is an enzyme found in food such as potato, liver and yeast!. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by -product of metabolism. The enzyme catalase speeds up the decomposition of hydrogen peroxide (2H O ). Hydrogen peroxide breaks down into water and oxygen. Equation : Catalse Hydrogen Peroxide Water + Oxygen Catalse 2H O 2H O + O It is able to speed up the decomposition of Hydrogen Peroxide because the shape of it matches the shape of the Hydrogen Peroxide. This type of is called an anabolic reaction as the Hydrogen Peroxide molecule is broken up into smaller peices. ...read more.

Middle

This has also been shown to be true of reaction catalysed by enzymes when other conditions, such as concentration of enzyme and substrate, are constant but it is important to remember that this will only occur between approximately 4 degrees and 50 degrees Celsius. Prediction : I believe that the rate of production of O and H O will increase, with substrate concentration. This rate is most probably proportional, until the saturation point is reached. After the saturation point has been reached I believe that adding extra substrate will make no difference. I also think that the more substrate that is being used, the more enzyme will be used, so therefore the amount of O that will be given off will be higher. The rate will steadily increase when more substrate is added, this is because there will be more molecules of the enzyme that are being used which will therefore result in more reactions so the amount of oxygen is produced more rapidly. ...read more.

Conclusion

6. Repeat the experiment, exactly the same, but using hydrogen peroxide concentrations of 80%, 50%, 40% and 20%. 7..As with the 100% concentration, repeat all the tests at three more times so that an average can be obtained. Repeating the experiments several times will help to produce better and more accurate results. Results: Time Taken Grams Of Yeast % Of Concentration (2H O ) Amount of 2H O (ml) 1st 2nd 3rd 4th Averages 0.2 g 100 4 17.91 19.05 18.91 17.32 18.29 0.2 g 80 4 1min33 1min47 1min36 1min44 1min40 0.2 g 50 4 3min22 3min49 3min32 4min01 3min53 0.2 g 40 4 4min19 4min31 4min27 4min41 4min29 0.2 g 20 4 6min32 5min46 5min50 6min03 6min22 (Results table) The times above are in minutes/seconds. The average times that I have worked out for each concentration are showed in the far right-hand column, I have worked them out to two decimal place. From the results I have found above I was able to plot various graphs of the rate of reaction against the concentration of Hydrogen Peroxide. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. To investigate the rate at which hydrogen peroxide is broken down by the enzyme ...

    table, drawing graphs of my results would be much clearer, with the aid of a line of best fit, once I have completed all the graphs. If, however, there is any anomalous data within certain concentration categories, the averages of the concentration categories should even these out and create a good line of best fit.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    The 75% Catalase concentration line had a slight increase in rate of reaction at the third minute; this line does not follow the pattern so the standard deviation calculation did not get rid of every anomalous result. I predicted that "at any given enzyme concentration, whilst the substrate amount and

  1. Reaction of Catalase and Hydrogen Peroxide

    This in turn will affect the number of enzyme-substrate complex being formed, which will affect the rate of reaction. 7. Temperature The temperature of a system is to some extent a measure of the kinetic energy of the molecules in the system.

  2. Investigating the effect of the Temperature on the Enzyme Catalase when it reacts with ...

    So therefore if I was to record the temperature at 60?c it would be approximately 0.82s. If the enzyme catalase does not denature at these temperatures then this suggests that I will have to investigate further on by using higher temperatures to find out at which temperature the enzyme will denature.

  1. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    Enzymes are made up of amino acids. Each amino acid has a -NH2 group and a -COOH group, as Ill as certain amino acids that have an extra -COOH group (e.g. aspartate) or an extra -NH3+group (e.g. asparagine). pH is all about concentration of H+ ions.

  2. To Investigate (By Experimentaion) the Effect of Substrate Concentrations On the Rate of the ...

    The photorespiratory pathway is shown on the right. This shows how hydrogen peroxide is produced and the functions of the peroxisome as part of the photorespiratory pathway. Non-specialized peroxisomes are a third group, which are found in other tissues. Potato tubers contain peroxisomes, although I don't know why.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work