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An investigation into how the enzyme lipase reacts under different atmospheric conditions.

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Introduction

An investigation into how the enzyme lipase reacts under different atmospheric conditions. Preliminary Work: Lipase is a digestive enzyme, which speeds up a reaction (catalysts). It speeds up the breaking down of fats into glycerol and fatty acids: FAT --> Glycerol + Fatty Acid (Neutral) (Acid) As on the other hand phenol red is an indicator, which detects the degree of how acidic or alkaline a particular substance is. Alkaline / Acidic (Pink) (Yellow) The final solution used in this investigation will be Sodium Carbonate, which is an alkali solution. This will be used to simulate a slightly alkali condition for the enzyme to work under, therefore the solution will be artificially alkali. Preliminary Results: From the results of my preliminary investigation I have produced a table of its results. Temperature (�C) Start Time End Time Length Of Time Taken (min) Room Temperature 19�C 3:15 3:37 22 Minutes 40�C 3:20 3:30 10 Minutes 87�C 3:18 No reaction ? From the results of my preliminary investigation I have chosen the following temperatures to be studied in my main investigation: 20�C 30�C 40�C 50�C 60�C 80�C I have chosen these temperatures as I feel that they will provide me with a good spread of data. ...read more.

Middle

Used to change solution artificially alkali. 21 drops (approx) How to make this a fair investigation: During this investigation there are many factors that could affect the overall results of the experiment, this would therefore make the test unfair. For the duration of this investigation I will only change one factor to make this a fair test, I will change the temperature of the water baths which will show me how the enzyme, lipase performs under various conditions. I will be required to reduce the effects of the following factors as they may influence a change in the results: The concentration of solutions (Milk, Lipase, etc) The amount of solution The equipment used (Size shape etc) To keep the previous list of factors, less of a problem and to create a fair test I will use the same concentration of solution and the same amounts of solution throughout the investigation. To keep the equipments size, shape, and surface area the same I will use the same equipment throughout the experiment and rinse thoroughly between each test. To prevent the use of mistakes in my investigation I will produce 4 repeats at each temperature. I will then find the average of the total list of results, which will provide me with more accurate results; this will also prevent any bias. ...read more.

Conclusion

As soon as the 'enzyme-substrate complex' has formed the products of the reaction are released and the enzyme is ready to accept another substrate molecule. This was shown as the reaction took place inside the test tubes at the various speeds. The speed of the reaction would depend on how quickly the 'enzyme-substrate complex' is produced and how quickly it can move on to another substrate molecule. Evaluation: I thought that the results that I found were fairly accurate to the same degree of accuracy as the equipment, as the equipment that was available was not the most accurate. So this is one of the factors that I would change if I were to repeat this experiment or a similar experiment in the future. I would also use more accurate water baths as the available electrical waters are well known for their inaccurate readings. I would also increase the amount of experiments taken at each temperature and the amount of temperatures tested overall. I believe that the final factor that I could change that would increase the accuracy of my results is that if I were to have a longer time to conduct the experiment I would complete the experiment one at a time which would reduce the amount of human error made. All of the results that I have found and produced have all fitted into the pattern that I would have expected before the initial investigation. ...read more.

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