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An Investigation into the affect of the concentration of the enzyme Neutrase on a solution of Marvel (dried milk powder).

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An Investigation into the affect of the concentration of the enzyme Neutrase on a solution of Marvel (dried milk powder) Introduction Neutrase is an enzyme. Enzymes are biological catalysts which means they speed up the reaction rate of substrates by up to a million times. They are made of protein (chains of amino acids which are folded into complex shapes) and are sensitive to pH and temperature. Enzymes only work on substrates. Different enzymes break down different food and the enzyme neutrase breaks down marvel (dried milk powder). A main part of the enzyme is the active site. The active site is shaped to fit its substrate. The substrate fits into the active site like a key into a lock. pH affects the enzymes because each enzyme has an optimum temperature. The reaction would be slower is the pH was further than the optimum pH. Denaturing often occurs when the pH is too far apart from the optimum pH. Another key issue that changes the reaction rate of the enzyme is its temperature conditions. The optimum temperature is around 45?C. ...read more.


The scientific reason for this is that if there are more particles, there is a higher chance of the particles colliding, therefore a higher reaction rate. So halve the particles double the time. Method of Experiment * Set up equipment as shown above (the prediction). * Draw a cross on the bottom of the beaker, as shown in the "above view" * Measure out 10 cm3 of marvel into a measuring cylinder and then pour it into the small transparent beaker. * Use a pipette to place 2cm3 of marvel into the beaker. * As soon as all the neutrase goes into the beaker, shake the mixture (with your thumb covering the end) and then start the stop clock. * When the marvel had is clear enough to roughly see the cross on the bottom of the beaker. Stop the stop clock. * Then repeat the experiment with neutrase concentrations of 1.00, 2.50, 5.00 and 10.00. Do each concentration three times to get accurate results. * When finished wash up all the apparatus. If you get several odd results then do that/those particular experiments again, and be more careful. ...read more.


There are no results that do not fit the pattern we see. There are no anomalies. It was possible to have used a bigger variation of the concentration of neutrase. I should have measured (for each percentage) the pH to make sure it was the same throughout the whole experiment. The enzyme was stored at roughly room temperature every time it was used, but to make it more accurate the temperature should have been measured every time it was used, but as we did all the results in one lesson the temperature would have only gone up or down within 2 degrees of the starting temperature. The temperature should stay the same because of the fact that enzymes denature at about 45�C. For accuracy better equipment should have been used. To make my own experiment more reliable I could compare my results with my fellow classmates. I could have found out the effects of protease (which is another enzyme) on Marvel and compare the two enzymes and see what similarities and differences we get in the results. The enzymes work better in a temperature of about 30 �C so we could do the experiment at different temperature and see what results we get. Hannah Lawson-West Biology SC1 1 1 Oakham School Science Investigation 09/05/07 ...read more.

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