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An investigation into the effect of temperature on the activity of enzyme catalase.

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Assessed Practical Name: Behrooz Khoshooee Student Number: 283644 Biology, December 2005 Title: An investigation into the effect of temperature on the activity of enzyme catalase. Introduction: We will try to demonstrate the effect of temperature on the activity of enzyme catalase and how this would affect the rate of activity. Hypothesis: Increasing temperature would increase the active energy in molecules and it would increases the rate of reaction. However too high or too low temperature would denature the enzyme and resulting in lowering rate of reaction and maybe no reaction at all. Methods: Filled 15 test tubes with 5 cm depth of hydrogen peroxide. Used ice bath to take these tubes to the required temperature under the room temperature. ...read more.


0 0 0 Conclusion: As we expected the increase in temperature increased the rate of reaction till it was higher than optimum temperature were the heat would start to denature the enzyme and it would slow down the activity. Discussion: Enzymes are substance that speed up a chemical reaction and are used in body to lower the activation energy. Without enzymes none the chemical reactions that happen in cell would have happened in normal cell condition. Enzyme work by binding to the substrate at active site, this is called lock and key. So if the active site change shape means that enzyme can't bind with substrate and there wont any reaction. Each enzyme has a specific active site shape which makes it specific for one reaction and wouldn't be any use in other reactions. ...read more.


So it can be seen in the graph that the high and low temperature has denatured the enzyme and resulting in slowing down the reaction but in between the increase of temperature increased the rate of reaction till the optimum temperature. The temperature that the rate of reaction would stop increasing by increasing the temperature and would start going down again is called "optimum temperature". One of the problems that we had while doing this experiment was, once we made sure that we achieved the right temperature than we took the test tube out of ice bath or water bath. This resulted that the hydrogen peroxide wouldn't have the right temperature while doing the experiment and not getting good result. So we had to repeat some of the tests but this time make sure that hydrogen peroxide reaches the required temperature and it stays in that temperature while counting the bubble. ...read more.

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