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An investigation into the effect of temperature on the rate of reaction of yeast.

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An investigation into the effect of temperature on the rate of reaction of yeast. Aim My aim is to view the effects of different temperatures on the rate of respiration of yeast in a glucose solution. I will do this by measuring the rate of which carbon dioxide is given off (CO2) by the yeast. Scientific knowledge From previous experiments I have learnt several things about yeast and enzymes. I have learnt that an enzyme is a natural catalyst, (something that speeds up the experiment without altering the out come.) Yeast is a fungus that is used in fermentation this is because it respires anaerobically and aerobically, the anaerobic respiration is the useful bit in fermentation as it produces ethanol (alcohol). The sucrose solution that the yeast is put in is needed as energy so that the enzymes in yeast can respire. The equation is: Glucose Carbon dioxide + Ethanol From a similar experiment with amylase I have seen that a change in temperature can drastically change the rate at which yeast respires. This is because of a theory, which is called the "collision theory" this says that when something is heated then it will move faster thus there will be more collisions between the substrates and the enzyme (glucose and the yeast). ...read more.


From my preliminary work I also found that by doing more than one repeat I could get a more accurate result and at the same time I could eliminate anonymous results. I have also seen that there is not enough gas at temperatures below 20?C to measure with the syringes we have been provided. I have also seen that temperatures above 70?C do not change the amount of gas at all, there is none being produced. I have found that it the temperature of the water bath goes down and therefore the temperature of the solution is going down. I am going to have to add hot water from the water bath to keep the temperature the same through out the time; re-activation time and timing time. I managed to make a table of the results from the preliminary work: Temperature /?C Volume of CO2 per 2 minute interval/ cm3/2 min-1 Run 1 Run 2 Run 3 Average 30 4.6 5 4.8 4.8 50 5.1 6.1 5.7 5.3 70 0 0 0 0 Range of conditions I have chosen the range of 20?C to 70?C as I have seen from my preliminary work that temperatures either side of this band are of no use to the experiment. ...read more.


> Then I put the delivery tube so that the bubbles of CO2 went into the syringe of water, and so pushing the water out and measuring the amount of CO2 produce. > I timed 2 minute and recorded the amount of CO2 produced. I did this 3 times. > I then did it at the other temperatures and repeated the method as above. > I recorded my results in a table of results. Safety Before starting there are a few safety aspects, which I have to be careful of. Firstly I need to be careful of the water, as some of it is very hot. Obtaining A table to show the results from an experiment into the effect of temperature on the rate of reaction of yeast. Temperature /?C Volume of Carbon dioxide per 2 minutes / cm3/2min-1 1st run 2nd run 3rd run Repeat of anomalous average 20 2 1 2 - 1.6 30 5 4.5 5 - 4.6 40 2.5 7.5 7 7 7.2 50 5 6 6 - 5.6 60 1.5 2.3 2 1.9 2.1 70 0 0 0 - 0 All anomalous results were repeated and not used in working out the average amount of carbon dioxide given off per 2-minute interval. The anomalous results are highlighted in the table in red. The figures in the average column are to 1 significant figure. Analysis Daniel Pitt 27/04/07 Biology SC1 page 1 ...read more.

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