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An Investigation into the Effect of the Catalase enzyme on Hydrogen Peroxide.

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Introduction

An Investigation into the Effect of the Catalase enzyme on Hydrogen Peroxide PLAN I am going to investigate the effect of the catalase enzyme on hydrogen peroxide, using starch for the catalase. Catalase is a biological enzyme which works best between 30 and 40 degrees, like most enzymes. It also has an optimum pH. Catalase breaks down Hydrogen Peroxide into water and oxygen. Plant and animal cells contain catalase. Variables Independent variables are things that I can change. Possible independent variables for this investigation are: � Temperature - the rate of reaction depends on the temperature as enzymes work best at certain temperatures. � Concentration of Catalase (enzyme) - the more concentrated the quicker the reaction. � Concentration of Hydrogen Peroxide - the more concentrated the quicker the reaction. � pH - enzymes have to be at the right pH to work properly. Dependent variables are things that change because of what I do. In this investigation possible dependent variables are � Volume of gas given off / evolved � Time I have chosen to measure the volume of oxygen given off over a period of 2.5 minutes, every ten seconds. I will make my investigation a FAIR TEST by controlling variables: � Concentration - always using 10ml of Hydrogen Peroxide. ...read more.

Middle

8) Then I put the bung in the test tube and immediately started the stopwatch. 9) Every ten seconds for 2.5 minutes I recorded how much gas had been made. I will repeat each test once. Amount of starch I will use is Starch Water 10ml 0ml 8ml 2ml 6ml 4ml 4ml 6ml 2ml 8ml Preliminary Experiment Before I started my main investigation I needed to know what a suitable range of water to use and how much Hydrogen peroxide and catalase to use. Here are my results for 10ml H2 O2 + 5ml starch + 5ml water Time (ins) Oxygen evolved (cm3) 0.5 1 1 3 1.5 4 2 4 2.5 5 Here are my results for 7ml H20 + 3ml Starch Time (ins) Oxygen evolved (cm3) 0.5 2 1 3 1.5 4 2 6 2.5 8 From this experiment I decided that I needed to use more starch for a quicker reaction and I decided that I should use 10ml of H2 02. I also decided to carry on my experiments, instead of using five minutes I chose 2.5 minutes. Obtaining evidence I obtained my results by carefully following my plan and method. The results I obtained were as follows: 21C Time (SCEs) ...read more.

Conclusion

There is one anomalous result for 6ml starch and 4ml water. This could be due to the fact that the stopwatch was misread or the bung might have been forced off the test tube during the experiment so that no gas was collected. My first graph shows that the optimum amount for catalase is10ml. This is because at 2ml the enzyme became denatured and could not form an enzyme substrate complex, therefore it produced no oxygen. At 4ml and 2ml there was less oxygen evolved because the water has diluted to and slowed the reaction by allot the Hydrogen Peroxide molecules had less kinetic energy and so there were fewer collisions and fewer enzyme-substrate complexes were formed, so less oxygen was evolved. Evaluation My results are really reliable and accurate as they prove my prediction, which was that, the catalase would break down the hydrogen peroxide down best when there was no water. My results are good enough to support a firm conclusion and my conclusion is safe. I have already identified anomalous results in the previous section. In my investigation I think the following areas should be improved next time: � More repeats � Checking bung regularly � Using a more reliable stopwatch. Overall, I think my investigation was successful. ...read more.

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