• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

An Investigation to determine changes of membrane permeability in beetroot cells.

Extracts from this document...

Introduction

An Investigation to determine changes of membrane permeability in beetroot cells Aim: To witness the changes of the solutions due to the release of coloured enzymes in the beetroot cells, in order to monitor the changes in membrane permeability. This would be done by, using a colorimeter tube to measure the amount of red colour that diffused out of the beetroot. Hypothesis: Several pieces of beetroot of the same length are being tested at different temperatures to see, which beetroot will allow the most enzymes to diffuse out. Referring to the factors that affect the rate of diffusion it can be argued that the piece of beetroot undergoing the test at the highest temperature, which in this case is 80ºc would allow the chemicals to diffuse out of the beetroot faster than if they are tested at lower temperatures. ...read more.

Middle

In other words molecules at a higher temperature will diffuse faster than molecules at a lower temperature at a same given time. A Table Of The Amount Of Colour That Diffused Out Of The Beetroot Cell At A Given Temperature Temperature ºC Time in water bath (min) Time in deionised water (min) Colour diffused (%) 80 1 30 21 60 1 30 71 40 1 30 92 20 1 30 49 5 1 30 82 Conclusion: The results for the practical does not relate to the data quoted in the hypothesis. In the hypothesis it was stated that the graph would show a gradual depreciation from the left side to the right, but what has actually happened is that the graph has shown no correlation. ...read more.

Conclusion

For example a beetroot left in a water bath at 60ºc for over a minute could have had more colour diffused out than a beetroot in a water bath at 80ºc for less than a minute. This happened because at times the stopwatch was started at the wrong time, and the beetroot was removed from the water bath late. From looking at the graph it cannot be stated that there are any anomalous results, since there is no correlation on the graph to suggest that one result is anomalous, while the other is not. The conclusion cannot really be reliable since the results are completely unreliable. The conclusion concludes that the hypothesis is correct but since this was not put into good practice there is no data to back it up. Felix Adams ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    An investigation to examine the effects of temperature on membrane stability in beetroot, by ...

    3 star(s)

    To avoid this happening, I should wipe the sides of the cuvette with a paper towel to make sure that there is no condensation on the sides and so it cannot affect the colorimeter readings that will be obtained. There may have been fingerprints on the sides of the cuvette that had no ridges.

  2. Marked by a teacher

    Beetroot Practical Write up

    3 star(s)

    experiment as this was the same filter used in the preliminary experiment. 5. Accuracy: No spillages or loss of solution from splashes as this would affect the volume and ratio of the ethanol and distilled water 6. Diameter of beetroot: From preliminary results the diameter of the beetroot was changed

  1. Marked by a teacher

    AN INVESTIGATION INTO HOW TEMPERATURE AFFECTS THE PERMEABILITY MEMBRANE OF A BEETROOT.

    3 star(s)

    the polypeptide chains together (like hydrogen bonds, sulphur bridges and ionic bonds) can be disrupted very easily- this damages the vacuole and makes holes in the cell membrane, inducing more leakage of the betalain pigment. Observing the results, at 54°C to 67°C, the percentage light transmission tends to be either

  2. Marked by a teacher

    The effect of temperature on the permeability of beetroot membrane

    3 star(s)

    The confidence limits also show if the results are significant or insignificant. I will also be using standard deviation which shows me the variation between the results. The lower the standard deviation the more accurate the results. * Decide how much replication you will need for successful analysis I will

  1. Marked by a teacher

    Effect of different temperatures on the permeability of beetroot cell membrane.

    3 star(s)

    This means it is over the critical value (0.79) therefore there is a positive significant correlation between the two values. Analysis Conclusion In my two graphs the overall trend is when the temperature is increased the absorbance of the beetroot is increased. At no point in my two graphs do you see the absorbance decreases when the temperature is increased.

  2. Permeability of Cell Membrane in Beetroot Cells

    greater the increase in heat, the greater the energy of the molecules. The more energy the molecules have the more they move around, this is how the pigment leaked out. At lower temperatures, the molecules were moving around slowly so there was very little leakage, however, when the temperature increased

  1. Catalyse Investigation

    I began with an enzyme concentration of 15 potato discs rather than a lower quantity to ensure that the apparatus was working correctly. Vol. pH buffer (ml) Vol. Hydrogen peroxide (ml) No.Potato discs 5 5 5 5 5 10 5 5 15 5 5 20 5 5 25 5 5

  2. Amylase Investigation

    * Instead of using 0.5cm3 of amylase, use 1cm3. This will speed up the experiment slightly because before the amylase was mixed with 5cm3 of starch and it took longer to digest the starch. Also other preliminary test on the effect of pH on amylase activity suggests that 1cm3 of

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work