• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Anaerobic Respiration In Yeast

Extracts from this document...

Introduction

Anaerobic Respiration In Yeast Christopher Finch Christopher Finch Anaerobic Respiration In Yeast Aim: I am going to investigate how changing the concentration of glucose affects the affectivity of the enzymes in yeast. I will be measuring the amounts of carbon dioxide expelled in the space of 4 minutes. Prediction: I predict that the enzymes in the yeast would react faster as the temperature increases up until a certain temperature, which would cause the enzymes to denature. This is mainly because if the temperature of an enzyme rises to or past a certain amount the tertiary structure of the protein is affected. The molecules begin to clump together and therefore can no longer effectively control the speed of a reaction. This de nurturing usually occurs at a temperature above 45 degrees Celsius as with proteins. I also predict that the concentration of the solution (glucose) would also affect the reaction speed. Using a concentration of glucose, which is too high, would induce plasmolysis to occur. Which would stop the reaction completely. This occurs when (in the case of this experiment) the glucose solution is so highly concentrated that there is more water present in the cytoplasm of the yeast cells than there is in the glucose solution. Osmosis then takes place, the water passes out of the cell into the glucose solution causing the cell to crenelate and so it becomes plasmolysed. ...read more.

Middle

There will not be enough available time for this variation of the method to be carried out. Results: 1st Set of results Glucose concentration 1st minute 2nd minute 3rd minute 4th minute 1% 3 2 3 6 2% 3 3 4 5 3% 5 4 4 3 4% 9 0 0 0 5% 6 5 6 10 6% 6 7 13 11 7% 12 15 17 18 8% 5 9 15 8 9% 4 9 12 12 10% 3 6 8 3 2nd Set of results Glucose concentration 1st minute 2nd minute 3rd minute 4th minute 1% 2 3 5 5 2% 3 2 5 4 3% 4 6 4 5 4% 5 4 6 6 5% 6 5 5 4 6% 7 8 7 13 7% 11 16 15 19 8% 6 6 4 8 9% 4 5 5 0 10% 2 0 3 1 Control: * The control is needed so that the reaction between water, which should not make the yeast react, and boiled yeast which, should prove that above a certain temperature the yeast will de-nature. Solution 1st minute 2nd minute 3rd minute 4th minute Water 3 2 1 2 Optimum Glucose Solution (7%) + Boiled Yeast 0 0 0 0 Averages: Glucose concentration 1st minute 2nd minute 3rd minute 4th minute 1% 2.5 2.5 4 5.5 2% 3 2.5 4.5 4.5 3% 4.5 5 4 4 4% 7 2 3 3 5% 6 5 5.5 7 6% 6.5 7.5 10 12 7% 11.5 15.5 16 18.5 ...read more.

Conclusion

As predicted no bubbles of any kind were expelled. Evaluation: The results are quite accurate, as two different sets of methods have been used to compare both. There were slight changes such as the temperature of the glucose, but these were only very minuet. The results found using my second method where different to that found using the first method. This may have been due to small variables; if I were to repeat the experiment I would try and eliminate all of the variables. The results I gained all seem to back up my conclusion and prediction. The results show that increasing the concentration of the glucose would cause plasmolysis to occur and so slow of even stop the reaction. The odd result where no reaction took place proved that the yeast would de-nature at higher temperatures. If I were to do the experiment again I would also use a gas syringe so as to be able to accurately measure the carbon dioxide given off instead of counting bubbles which could have been different sizes so counting the bubbles is not an accurate way to measure carbon dioxide being given off. I would also make use of catalysts, which speed up or slow down the reaction, as a catalyst often provides a surface where particles can meet to react, reactions on these surfaces use less energy so they often allow reactions to work at lower temperate saving fuels. It would also be better for my averages if I were to repeat the experiments more times to be able to find more accurate averages. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Exchange, Transport & Reproduction section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Exchange, Transport & Reproduction essays

  1. Peer reviewed

    "An investigation into the Respiration of Carbohydrate Substrates by Yeast."

    5 star(s)

    The results which I feel are anomalous are highlighted in the table above. They have not been excluded in order to provide accurate statistical analysis. My own results are highlighted in red. I think that both my own and the group results are fairly accurate as they are in keeping with the information gathered before the pilot i.e.

  2. Investigating Anaerobic Respiration in Yeast

    I chose to investigate this because I think it will generate some clear and meaningful results that can be re-tested easily.

  1. An investigation to see whether the concentration of Sucrose effects the amount of Carbon ...

    The 20% concentration line is roughly the same steepness (if not more gentle sloping) as the line representing 15% concentration. The steepness of the line represents the rate of reaction. If the line is steep it means that the rate of reaction is quick and if it is a gentle slope this means that the rate of reaction is slower.

  2. Rate of Respiration

    * Stop clock * Safety goggles * Rubber stopper * Distilled water * Electronic balance * Beaker * Universal indicator * Acidic buffer solution * Funnel * Buffer Solution Method of investigation Steps Accuracy Reason for method All apparatus that will be used to contain yeast or sugars will be

  1. The Effect of Different Substrates on the Rate of Respiration on Yeast (Saccharomyces cerevisiae).

    12 1110 12 1140 12 1170 12 1200 12 Table 4 There are no results for the trial experiment for lactose because no gas was produced.

  2. Design an experiment to investigate the effect of temperature on the movement of a ...

    When I use it I will cut the beetroot in a smooth motion away from my body, and I will cut it on a ceramic tile. I have researched what I should do if I cut myself. (source- http://www.medicinenet.com/cuts_scrapes_and_puncture_wounds/article.htm) The most important first step is to thoroughly clean the wound

  1. Rate of respiration in Yeast.

    will waste the energy of the yeast as it has to break down the large molecules into smaller molecules before it can use them. This means that the sucrose is not as efficient as the glucose at providing the yeast with a better medium by which it will produce a faster rate of respiration.

  2. Investigate the effect different concentrations of glucose in a yeast & Glucose solution has ...

    Heavy metals such as Zinc * Stirring * Volume of yeast + Concentration * Volume of glucose Some of these factors will be controlled while others will be changed, or measured. Underneath you will see a table of what will be done.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work