Aseptic Conditions

Aseptic condition is a phase used to describe that the area you are in must be thoroughly clean as in things such as the tables, food, hands and even the air. An good example would be an scientist who is about to undergo an experiment, he must firstly clean the area he is in so that nothing goes wrong in the experiment but this is only depending on the type of experiment your doing. The scientist must clean all the equipment such as the lab mats, beakers, and he must make sure he washes his hands also; this is meant by aseptic condition it is basically to just clean the area within you when carrying out a certain task.

When you’re in an aseptic condition it must not be possible for any bacterial contamination of a product to occur during the experiment whatsoever.

The word Aseptic means "without micro organisms." Aseptic technique refers to practices that help reduce the risk of infections in people by decreasing the possibility that micro organisms will enter or harm the body during certain procedures.

Aseptic techniques are those that do some or all of the following:

Removes or destroys bacteria from the surface of your hands and objects.
Reduces the chance of getting micro organisms that cannot be removed.
Occupy disinfected ...

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When you’re in an aseptic condition it must not be possible for any bacterial contamination of a product to occur during the experiment whatsoever.

Aseptic techniques are those that do some or all of the following:

Removes or destroys bacteria from the surface of your hands and objects.
Reduces the chance of getting micro organisms that cannot be removed.
Occupy disinfected instruments and other Items.

Examples of aseptic techniques would be:

Washing hands (Cleaning with soap and water)
Surgical scrub
Using safe operative methods (making small incisions and so on)
Ensuring your in a safe environment in the procedure area.
Sterilisation (Eliminates all micro organisms)
Provide clean air in the working area

When using sterilisation as an aseptic technique there are many ways in which it could be done, one for example is using steam. Steam sterilization (frequently referred to as autoclaving) depends on the use of steam above 100oC it can only be used with certain materials and certain metals, if it was to be used on a plastic it would cause it to melt because of the high temperatures. But some materials are sterilised through extreme temperatures, for instance when you’ve just completed an experiment and you need to sterilise the wire loop from any bacteria, you just simple heat the wire loop over the Bunsen burner and this will instantly kill the bacteria.

Types of Aseptic Techniques are as followed:

Autoclaving- Autoclaving, or sterilising using steam under pressure is a good way of killing off bacteria and unwanted organisms. The temperature inside the autoclave depends on the pressure outside of the autoclave, the greater the pressure the higher the temperature. The autoclave is used for heat resistant equipment which needs to be sterilised.

Hot Air Oven- This method of sterilisation uses dry heat that requires high temperatures for efficient sterilisation than moist heat does. This method is used for more delicate equipment such as syringes.

Infrared Radiation- This method is also used for delicate equipment just like the hot air oven, the infrared rays heat the instrument up to really high temperatures killing off all organisms that exist on it.

Gamma Rays- The method used for gamma rays sterilisation is that the organisms that exist on the equipment will be blasted with some gamma radiation, this will then disturb the normal function of their cells and it would kill them automatically, this method is often used for materials that are easily damaged by heat.