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Biology Assessed Practical -The Effect Of Temperature On Enzymes.

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Biology Assessed Practical-The Effect Of Temperature On Enzymes Hypothesis We are going to test the effect of temperature on enzymes. We will test the enzyme at 7 different temperatures. Out of these temperatures, we have to test at least 5. The temperatures are: 0�C, 20�C, 30�C, 40�C, 50�C, 60�C and 80�C. We are only going to use one types of enzyme. The enzyme we are going to use is called Amylase. This enzyme acts on Starch so we are going to test how long it takes for the enzyme to break down the starch when set at different temperatures. According to the sheet " How Enzymes Work" given to us in an earlier lesson, the enzyme should work best at around 40�C. It should also not even work after around 55�C, as the enzyme will become denatured and will not be able to work properly. My prediction is that the enzyme will work very slowly on the temperatures 0�C and 20�C, it will work faster on 30�C and 50�C, it will not work at all (or very slowly) on 60�C and 80�C, however, will work best on 40�C (around body temperature). The reason I think the enzyme will work this way is because amylase is found in the human body, which is usually around 37�C. ...read more.


* After every 0.5 mins stir the mixture with your glass rod, put a drop of the mixture onto one of the Iodine filled dimples in the dimple tile and clean the glass rod in the beaker of water. * Record whether there is starch present in your results table and keep testing every 0.5 mins until the Iodine solution does not change when the mixture is added to it. * When you have done this test, clean out your two test tubes and dimple tile and repeat using a different temperature. * When that test is done repeat for another three temperatures. * N.B. If the starch is still present after 15 mins then stop the test. Results 20 �C Time taken (min) Starch present? 0 Yes 0.5 Yes 1 Yes 1.5 Yes 2 Yes 2.5 Yes 3 Yes 3.5 Yes 4 Yes 4.5 Yes 5 Yes 5.5 No 30 �C Time taken (min) Starch present? 0 Yes 0.5 Yes 1 Yes 1.5 Yes 2 Yes 2.5 Yes 3 Yes 3.5 Yes 4 No 4.5 No 5 No 5.5 No 40 �C Time taken (min) Starch present? 0 Yes 0.5 Yes 1 Yes 1.5 Yes 2 Yes 2.5 Yes 3 No 3.5 No 4 No 4.5 No 5 No 5.5 No 50 �C Time taken (min) ...read more.


I trust that I measured correctly, as I had no air bubbles in my syringes, on all of the 6 experiments I did. However, some people may not have used the same concentration of Iodine as me, or more than I used. Also, I think that the whole of our class worked well together cooperating, seen as though we were all doing separate experiments from each other. I followed the whole of my safety rules regarding the experiment to make the environment I was working in more secure for my classmates and I. Furthermore, I followed the whole of my method, making sure that I didn't do anything wrong or made silly mistakes. If I was to do the experiments again, learning from my experience and mistakes, I would make sure that I did my experiments at the least crowded water bath, to make my experiment more accurate and safe. Out of the experiments that I did, the only experiment results that came as a shock to me were the 60�C and 80�C results. In my hypothesis I predicted that they would work very slowly or not at all and in fact in my experiment 60�C was the fastest result from both me and my class and 80�C was 4th fastest in my results and 5th fastest in the class results. Bethany Francis 10m ...read more.

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