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Biology Course work - Hypothesis : The higher the volume of lactase left to react with lactose, the shorter the length of time it takes to break it down.

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Candidate name: Katy Taylor Candidate number: 6110 Biology Course work- Hypothesis: The higher the volume of lactase left to react with lactose, the shorter the length of time it takes to break it down. Lactose is a sugar most commonly found in milk. Some people have an intolerance to this sugar and it is at this point it has to be removed. The reason for this intolerance to lactose is due to the inability to digest a significant amount of it. This inability the result of shortage of the enzyme lactase, which is normally produced by the cell lining in the duodenum can be quite painful. The importance of this enzyme lactase is that it breaks down the sugar lactose, so it is able to absorb into the bloodstream. The absence of it can therefore mean that when there is allot of lactose which can not be broken down it can cause a distressing problem. These problems can be such things as cramps, nausea, bloating, gas and diarrhoea. These symptoms can begin from just thirty minutes to two hours after consumption of lactose. This is therefore quite a distressing problem. The enzyme lactase can actually be obtained without prescription and is a very sufficient remedy when tending to lactose intolerant people, small amounts dropped into milk for a length of time can reduce lactose by up to seventy percent. ...read more.


into one of the test tubes holding the Benedict solution and place immediately into the 100 degree water bath. I will then wait to see what colour the solution will turn. When Benedict solution detects sugar it must be at a 100 degree temperature and it also will turn a green/brown colour. I will wait for a colour to become visible then record the result at 0 seconds. Then when it comes to 2 minutes I will repeat this test and continue every two minutes until the solution has turned a brick red colour, this will mean there is no lactose in the milk present. I will repeat this procedure with the other enzyme volumes. If my hypothesis is correct then the 6ml volume of enzyme should take the shortest amount of time to break down. This is because in comparison to the 1ml of milk it is a larger volume and there will be plenty of active sites to bind with. The one which should take the longest is the 2ml of enzyme as this will have the least amount of active sites. To keep this experiment fair I will take the following precautions: * Keep safety goggles on at all times * Keep hair tied back if it is long * Place the Bunsen burner and 100 degree water bath in a safe place where it can not be knocked over. ...read more.


This is due to the fact that there were less active sites for the substrate to bind with and so the substrate must wait its turn to be broken down, this wastes time. Evaluation Although my results vary at points they still give the same implication, that 6mls of lactase is more sufficient for breaking down 1ml of milk in the shortest time, these results prove that my hypothesis was correct. This investigation and the conclusion of it may be used to benefit lactose intolerent people as they will not have to wait such a long length of time to get milk suitable for them to drink. Instead of using the normal dose of lactase , used by the lactose intolerant user, it could be possible to double or even triple the amount of lactose broken down, making it more suitable for them to use. In future, if I ever repeat this investigation, things I could change to make this test more reliable are: * To use the same Benedict solution for all readings * Maybe use an electric water bath where the water would be a constant temperature of 40 degrees. I feel this would be useful as it was hard to read results and check the temperature was at a constant. This investigation worked at a reasonable level, the fact that all my readings were not similar made it slightly less successful than I would have hoped, but the hypothesis was still proved right. ...read more.

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