Biology Enzymes Coursework

experiment; I will only change the temperature. I will keep all these things the same,· The amount of yeast+ glucose and the amount of Methylene blue.· The amount of water used.· The apparatus, test tube, beaker, thermometer and timer.· I will use the same batch of methylene blue and yeast +glucose.I will keep all of these things the same in order to do a fair test.Scientific theory: I know that the more common enzymes work best at around 40.c. This is there optimum temperature. If this temperature begins to rise the reactions slow down, this will continue to happen until the enzyme is denatured. It becomes denatured at around 60.c, this happens because the enzyme becomes misshapen. This means that the enzyme will no longer work because it will no longer fit, the active site will be ruined. The enzymes react like a lock and key, only one lock fits the key. When the two join then you get the chemical reaction. When too much heat gets applied then the lock gets changed and so the key doesn’t fit the lock so there is no reaction. (Better explained in my picture)Key when joined the reaction starts,Active site (lock)This reaction will not work because the Key lock doesn’t fit the key. This will never work again.Active site (lock denatured)As you can see as soon as the lock gets denatured it wll no longer work.Preliminary work: I watched someone else do this experiment and saw how it was to be done. This helped me see how the experiment worked and how to do things correctly. I also have done a lot of work on enzymes in lessons and know how they work which has helped me understand this process.Results:Temperature in .c 1st time in minutes 2nd time in minutes 3rd time in minutes Average time20 30 40 50 10+ 60 10+ 10+ 10+ 70 10+ 10+ 10+ There are too many negative results because the enzyme is denaturing too quickly, so as I thought I would have to narrow my temperatures to, 20.c, 30.c, 40.c, 45.c, 50.c and 60.c. This is my new results table.Temperature in .c Time taken in seconds 1st time Time taken 2nd time Time taken 3rd time Average time in seconds20 532 542 524 532.666666730 304 252 314 29040 153 157 152 15445 147 139 141 142.333333350 256 259 251 255.333333360 1000 1000 1000 1000From these results I can see that my prediction was wrong because the optimum temperature for this enzyme was around 45.c. I think that this is true because you can tell from my table and my graph. I think that this happened because it wasn’t a natural enzyme it was a chemical one. This enzyme denatured at around 60.c.Conclusion: from doing this experiment I have found that the chemical enzymes work best at a slightly higher temperature than the natural enzymes do. You can tell that this is true by looking at my graph or table. The chemical enzyme that I used, methylene blue, worked best at 45.c unlike the natural enzymes that work best at around 40.c.Evaluation: if I were to do this experiment again I would use the most sophisticated equipment available because you can’t record things well enough yourself. I would use a constantly heated water bath that was always at the correct temperature and I would use an electric thermometer too. I would do this and I would make sure everything was perfect before every temperature was recorded. I would also change my variations in the temperature so that I had a clearer idea of the exact optimum temperature. I would record no denatured results. These are all the changes that I would make.