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Biology Enzymes Coursework

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biology Prediction: I think that the enzyme will work best at 45.c to 50.c I think this because that optimum temperature for most natural enzymes is 40.c but his is a chemical enzyme so it will work best a little higher. If this temp is exceeded then I think that it will take longer to work because it will not be at the optimum temp, or it will not work at all because it has become denatured. An enzyme cannot recover from this state. Below this temperature I think it will be slowed down but will not denature because it can't at low temperatures only at high ones. These will not be to reliable so I may have to use a different set of measurements when I do it but for now it will be these. Fair test (how): I made this a fair test by only changing one thing in the experiment; I will only change the temperature. ...read more.


When the two join then you get the chemical reaction. When too much heat gets applied then the lock gets changed and so the key doesn't fit the lock so there is no reaction. (Better explained in my picture) Key when joined the reaction starts, Active site (lock) This reaction will not work because the Key lock doesn't fit the key. This will never work again. Active site (lock denatured) As you can see as soon as the lock gets denatured it wll no longer work. Preliminary work: I watched someone else do this experiment and saw how it was to be done. This helped me see how the experiment worked and how to do things correctly. I also have done a lot of work on enzymes in lessons and know how they work which has helped me understand this process. ...read more.


This enzyme denatured at around 60.c. Conclusion: from doing this experiment I have found that the chemical enzymes work best at a slightly higher temperature than the natural enzymes do. You can tell that this is true by looking at my graph or table. The chemical enzyme that I used, methylene blue, worked best at 45.c unlike the natural enzymes that work best at around 40.c. Evaluation: if I were to do this experiment again I would use the most sophisticated equipment available because you can't record things well enough yourself. I would use a constantly heated water bath that was always at the correct temperature and I would use an electric thermometer too. I would do this and I would make sure everything was perfect before every temperature was recorded. I would also change my variations in the temperature so that I had a clearer idea of the exact optimum temperature. I would record no denatured results. These are all the changes that I would make. ...read more.

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