• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Biology investigation to test for the presence of amylase in germinating seeds.

Extracts from this document...


Biology Report: Biology Investigation Maggie Ming F.5Sc (25) Hypothesis: a) Food stored is used up during germination for respiration for energy for growth by cells b) Food stored is consumed by micro-organisms Aim: To test for the presence of amylase in germinating seeds. Principle: a. Starch b. Hydrolysis c. Water + Starch amylase Maltose d. Amylase e. Action of amylase f. Amount of starch used g. Light sensormeter h. Temperature and pH i. A control is necessary. A control can be set up by replacing the fresh enzyme extract from the beans with boiled enzyme extract from the beans. The control set-up differs from the experimental set-up by the enzyme extract. Precautions: (1) Ensure that light from the surroundings will not interfere the results by covering up the 100ml plastic vial with a black cardboard paper. (2) Ensure that, in the control set-up, the bean extract is boiled thoroughly to denature all the enzyme inside. ...read more.


This explains why our aim for this experiment is to prove that there is the presence of amylase in germinating seeds, as glucose is the main energy releasing source and can be found in beans when starch inside beans are being digested by amylase. Since for Hydrolysis, Water + Starch amylase Maltose The activity of the enzyme extract in the fresh condition and the boiled condition was studied in this experiment. The method used to indirectly show this was the change in light intensity, which was observed through the sensormeter in each set-up. Because the fresh enzyme extract was being put into starch solution, which had been added with several drops of iodine solution and had turned blue-black, as the enzyme extract continuously digest the starch molecules inside the solution, the light intensity penetrating the solution will rise because the colour of the solution slowing fade and turns yellowish brown. This was what had happened in the experimental set-up. ...read more.


temperature) are the same for both experiments (3) Thicker black cardboard can be used so as to increase the difficulties for the light from the surroundings to penetrate through the paper and affect the results. (4) The black cardboard should also cover the table lamp and the battery powered light sensormeter to exclude any light from the surroundings. Conclusion: The hypothesis, which is "food stored in the beans are used up during germination for respiration for energy for growth by cells in the beans", should be accepted. To prove the second hypothesis, we can use the following set-ups. For the experimental set-up, we can use fresh beans, whereas for the control set-up, we can use sterilized beans. For the experimental set-up, we fill up half of a test tube and then connect the test tube to a closed container alkaline pyrogallol solution, and test for the changes in gas pressure using a pressure sensormeter. The same applies to the control set-up. If the second hypothesis is true, then there should be a greater increase in the pressure in the experimental set-up. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    An investigation into the inhibiting effect of tomato juice on the germination of cress ...

    3 star(s)

    So the tomato plants with the inhibitors will grow more and produce more offspring. Over a long period of time only those tomato plants with the inhibitors will survive to pass on their genes (the tomato plants with the variations are able to gain light, heat, etc, more easily; they are biologically fit.

  2. A Level Biology revision notes

    is placed on the pencil line to form a spot o Paper is fastened with a drawing pin to a bung o This is placed in a boiling tube with some amount of solvent inside o Spot needs to be suspended above the level of solvent!

  1. Catalyse Investigation

    H2O (CM3) H2O2 % Time (min) Length (CM) No. Of bubbles 10 150 15 5 3 2 20 150 30 5 3 3 30 150 45 5 3 3 40 150 60 5 3 4 50 150 75 5 3 6 Average No.

  2. Design an Experiment to Determine the Effects of Copper Sulphate Concentration on the Germination ...

    The seed can often double its mass within a few hours and this swelling causes the testa to burst. This is important as it enables oxygen to enter the seed. The action of water moving into the cell breaks dormancy and initiates metabolism by activating gibberellins.

  1. Amylase Investigation

    The synthesis involves hooking together 124 molecules in a very specific sequence to form the macromolecule. Such syntheses led to the probability of identifying those areas of the molecule that carry out its chemical functions, and opened up the possibility of creating specialized enzymes with properties not possessed by the natural substances.

  2. Spreadsheet report

    First I titled max (standing for maximum) and entered a formula at the end of the profits column. I clicked on the function button, selected the max function from the category, typed in the function and clicked ok. I did the same for the other three weekly tables.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work