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Biology investigation to test for the presence of amylase in germinating seeds.

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Biology Report: Biology Investigation Maggie Ming F.5Sc (25) Hypothesis: a) Food stored is used up during germination for respiration for energy for growth by cells b) Food stored is consumed by micro-organisms Aim: To test for the presence of amylase in germinating seeds. Principle: a. Starch b. Hydrolysis c. Water + Starch amylase Maltose d. Amylase e. Action of amylase f. Amount of starch used g. Light sensormeter h. Temperature and pH i. A control is necessary. A control can be set up by replacing the fresh enzyme extract from the beans with boiled enzyme extract from the beans. The control set-up differs from the experimental set-up by the enzyme extract. Precautions: (1) Ensure that light from the surroundings will not interfere the results by covering up the 100ml plastic vial with a black cardboard paper. (2) Ensure that, in the control set-up, the bean extract is boiled thoroughly to denature all the enzyme inside. ...read more.


This explains why our aim for this experiment is to prove that there is the presence of amylase in germinating seeds, as glucose is the main energy releasing source and can be found in beans when starch inside beans are being digested by amylase. Since for Hydrolysis, Water + Starch amylase Maltose The activity of the enzyme extract in the fresh condition and the boiled condition was studied in this experiment. The method used to indirectly show this was the change in light intensity, which was observed through the sensormeter in each set-up. Because the fresh enzyme extract was being put into starch solution, which had been added with several drops of iodine solution and had turned blue-black, as the enzyme extract continuously digest the starch molecules inside the solution, the light intensity penetrating the solution will rise because the colour of the solution slowing fade and turns yellowish brown. This was what had happened in the experimental set-up. ...read more.


temperature) are the same for both experiments (3) Thicker black cardboard can be used so as to increase the difficulties for the light from the surroundings to penetrate through the paper and affect the results. (4) The black cardboard should also cover the table lamp and the battery powered light sensormeter to exclude any light from the surroundings. Conclusion: The hypothesis, which is "food stored in the beans are used up during germination for respiration for energy for growth by cells in the beans", should be accepted. To prove the second hypothesis, we can use the following set-ups. For the experimental set-up, we can use fresh beans, whereas for the control set-up, we can use sterilized beans. For the experimental set-up, we fill up half of a test tube and then connect the test tube to a closed container alkaline pyrogallol solution, and test for the changes in gas pressure using a pressure sensormeter. The same applies to the control set-up. If the second hypothesis is true, then there should be a greater increase in the pressure in the experimental set-up. ...read more.

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