Biology SNAB Core Practical - Gram Staining Bacteria

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Core Practical - Gram Staining Bacteria

Introduction

When a bacterial infection is diagnosed antibiotics may be

prescribed. Different antibiotics are not equally effective

against all bacteria, so the correct antibiotic must be selected

for a particular bacterial infection. In some cases the most

effective antibiotic is known, but in other cases tests need to

be carried out by a pathology department. In this activity I

will be testing the effectiveness of several types of antibiotics

on bacteria

Hypothesis

The aim of the experiment is to test the effectiveness of different types of antibiotic on bacteria. The bacteria that we will be using will be Escherichia Coli and Bacillus Subtilis. I predict that the antibiotics will be more effective on B. Subtilis.

Variables

The Independent variable in this investigation is the presence of the different types of antibiotics. This will be changed by using a mast ring which has all the different types of antibiotics around it. The Dependant variable in this investigation is the zone of inhibition around the disc. This will be measured by measuring the zone around the disc, using a ruler, after the