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Catalase Investigation

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Introduction

Preliminary Experiment of the Enzyme, CATALYSE In this experiment I will be looking at the how hydrogen peroxide reacts with different materials to find out what happens to the enzymes in each material. I will be testing liver both raw and boiled a piece of apple and a piece of potato. I will put each sample into a test tube with 10ml of hydrogen peroxide then leave the experiment for 5 minutes I will then measure my results. Apparatus * Apple * Potato * Boiled liver * Raw liver * Test tube * Hydrogen peroxide * Scalpel * Tile * Stop watch * Test tube rack * Measuring cylinders * Goggles * Ruler Method 1. Cut a piece of apple using the scalpel and tile. 2. Put the apple piece into a test tube making sure it is at the bottom of the tube. 3. Using the measuring cylinder pour 10ml of hydrogen peroxide into the test tube. At any time when using hydrogen peroxide goggles must be worn. 4. At the same time as the hydrogen peroxide is added to the test tube and apple start the stopwatch and wait for 5 minutes. 5. After 5 minutes take the ruler and measure the oxygen bubbles on top of mixture. 6. Record the results into a table. ...read more.

Middle

Main Investigation After my preliminary investigation and looking at back ground knowledge I am going to alter the concentration of the enzymes Prediction I predict that the higher the concentration the faster the reaction will be and the products will be made. Because there is more of the hydrogen peroxide in a smaller volume of water so there is more collisions on the active site, to gain enough energy to over come the energy activation barrier and form oxygen and water faster. After a certain concentration the rate of the reaction will eventually stop increasing. This is because there is not enough yeast enzymes for the hydrogen peroxide molecules to react with and the reaction concentration will be come in excess Diagram Apparatus 10ml measuring cylinder Stopwatch Boiling tube Test tube Bung with delivery tube Hydrogen peroxide Water Yeast solution Plastic bowl Elastic band Clamp stand Flask Method 1. Fill the plastic bowl with water andand tie the elastic band around the test trube now fill the test tue up to the rubber band with water empty the water into a measuring cylinder and record the amount of water in cm3 2. Place the boilig tube in the clamp stand near to the bowl of water now add the 10ml of yeast solution to the boiling tube and insert the bung with the delivery tube going into the water. ...read more.

Conclusion

Before I did the next stage of the experiment I cleaned out all the apparatus this was to add more accuracy into the experiment. If I had sterilised the apparatus after each stage this would again increase the accuracy of the test but I do not feel this would have made a significant difference. Another experiment I could of done to look at the nature of enzymes is to of look at the effects of temperature on enzymes. If I used a water bath to control the temperature I could heat the hydrogen peroxide and the yeast solution and record hoe the temperature effects the reaction times. I predict that the reaction would of increased in speed as the temperature increased. I think this because the increase in heat energy which is given to the hydrogen peroxide and the yeast molecules will be transferred into kinetic energy this will make the molecules move around more and collide more often so the reaction will take place quicker. I think that a 5degree C increase in temperature each time starting form 5 and going up to 40 would be sufficient and if the temperature was to be raised any more then the enzymes would become denatured. This would be additional evidence that there are collisions between substrate hydrogen peroxide and the enzymes in the yeast solution. But after 40degree c the reaction time will start to decrease. ?? ?? ?? ?? Biology Course Work By Stuart Dobson 10KI ...read more.

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