• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Catalase Investigation

Extracts from this document...


BIOLOGY GCSE PRACTICAL ASSESMENT 1. Our investigation is to find the rate of catalase activity. Catalase is an enzyme, which in this case is used to break down Hydrogen Peroxide. SUBSTRATE ENZYME PRODUCTS 2H�0� Catalase 2H�0 + 0� Enzymes are catalysts, which means that they are used to speed up reactions. They are not changed or used up in reactions at all. They have molecules with a 3-D shape and they work best at about a pH of 7 because, as they are proteins, they are denatured by very acid and alkaline conditions. As they are proteins they are also denatured by high temperatures. They work quickest at their optimum temperatures (the best kind of environment suitable for them). It is the yeast that makes the catalase. Yeast is a living cell (it is a fungi). Inside the yeast hydrogen peroxide is produced and as it is toxic, the yeast also makes the enzyme (catalase) to break down the Hydrogen Peroxide (substrate). Catalase is located in the cytoplasm of cells. In the cytoplasm they control the chemical reactions occurring inside the cell. Enzymes are specific and so only one type of enzyme will only break down one particular type of substrate. ...read more.


3. This is the write up for my preliminary work. The reason for writing this is to give you an idea of what I am going to do and to tell you precisely how to do it. AIM The aim of this experiment is to find out the rate of catalase activity. SAFETY During the whole of this experiment I wore safety glasses for eye protection in case there happened to be a spillage and I got some hydrogen peroxide in my eye. As I was working in the science lab I had to follow the main lab rules, such as Do Not Eat Or Drink In The Lab. I also had to be very careful, as the hydrogen peroxide was corrosive. EQUIPMENT Here is the list of equipment that is needed to carry out this experiment CONICAL FLASK BEAKER SYRINGE BUNG 2 DELIVERY TUBES MEASURING CYLINDER 20 cm� HYDROGEN PEROXIDE 2 ml YEAST SUSPENSION (CATALASE) 200 ml WATER DIAGRAM METHOD I set up the equipment as it is shown in the diagram above. I first got a conical flask and put 20 cm� of hydrogen peroxide in it. The concentration of the hydrogen peroxide was 20 volumes, which was the maximum concentration I was allowed to use. ...read more.


* To vary the variable, which I chose, all I have to do is add twice the amount of water, which is already in the beaker, to the hydrogen peroxide. This will change the concentration of it and so if we add twice the amount of water to 100% concentration of hydrogen peroxide it will change to 50% concentration of hydrogen peroxide. * Each time I vary the variable I will be halving the concentration each time so it will first be 100% and then it will be varied to 50%, 25%, 12.5% and then finally 6.25%. * During the experiment I will be measuring how many seconds it takes for the mixture of catalase (yeast suspension) and hydrogen peroxide to produce oxygen and displace 25 ml of water in a measuring cylinder. * During this experiment I will wear safety glasses for eye protection in case there happens to be a spillage and I get some hydrogen peroxide in my eye. As I am going to work in the science lab I have to follow the main lab rules, such as Do Not Eat Or Drink In The Lab. I also have to be very careful, as the hydrogen peroxide is corrosive. * * * * Nadeem Arshad 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Beetroot Practical Write up

    3 star(s)

    time in one experiment and then at a shorter time the next, would not be considered accurate or constant. With the beetroot in at a longer length of time eventually the ethanol would have totally destroyed the cell membrane whatever the size of the beetroot or ratio with distilled water.

  2. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    At 100% concentration, the catalase molecules were in larger number, there were more catalase molecules relative to hydrogen peroxide molecules, so enzyme active sites were more readily available, keeping the rate of reaction high. If you look at the 'Class Average Graph', as the concentration of catalase molecules decreased the overall amount of oxygen gas produced decreased.

  1. Reaction of Catalase and Hydrogen Peroxide

    I will be tying my hair. 6. I will be keeping the solutions at distance from electrical devices. Method First I will make the 15% concentrated solution of hydrogen peroxide. This I will do by first taking 100ml of distilled water in the measuring cylinder from the bottle and pour into the beaker.

  2. The effect of Copper Sulphate concentration on Catalase activity on Hydrogen Peroxide.

    The aim of the investigation was to observe how the rate at which Catalase breaks down Hydrogen Peroxide with varying temperatures. Table to represent the volume of oxygen given off, at different temperatures: Temperature (�C) Experiment N�1 No. Of oxygen bubbles given off Experiment N�2 No.

  1. Catalyse Investigation

    Enzyme cofactors - cofactors are none protein substances which influence the functioning of enzymes. They include activators that are essential for the activation of some enzymes. Coenzymes also influence the functioning of enzymes although are not bonded to the enzyme.

  2. The aim of this investigation is to investigate the enzyme activity by using Catalase ...

    The active site of Catalase matches the shape of the Hydrogen Peroxide molecule; therefore Catalase can speed up the reaction. Catalase is an enzyme found in food such as potato and liver. It removes Hydrogen Peroxide from its cells, as Hydrogen Peroxide is a poisonous by-product of metabolism.

  1. Amylase Investigation

    Alpha amylase is resistant to comparatively high temperatures; its temperature optimum is 70�C (in mash to make beer) and it is destroyed at 80�C. It functions best at pH is 5.8. Beta-amylase is destroyed at 75�C and its optimum operating temperature is 60-65�C (in mash to make beer)

  2. Hydrogen Peroxide and Catalase

    Non-specialized peroxisomes are a third group, which are found in other tissues. Potato tubers contain peroxisomes, although I don't know why. I suppose if new plants have to grow from them, they have to have all the parts of the plant with the store of food.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work