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Catalase Investigation

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Introduction

Catalase Investigation Introduction: This investigation is to see how the concentration of hydrogen peroxide affects the rate of reaction between catalase and hydrogen peroxide. An enzyme is a catalyst that is found inside living organisms. Catalase is found inside the cells of living organisms, both animal and plants. Catalase breaks down the hydrogen peroxide that is produced in the cells through other reactions. Hydrogen peroxide is dangerous to the cells so it has to be broken down by the catalase quickly. The equation for what catalase does is: Hydrogen Peroxide + Catalase = Water + Oxygen. 2H2O2 = 2H2O + O2 An enzyme is made of protein and is designed specially to break down or to make one type of molecule. An enzyme has an active site into which its substrate fits exactly. The active site of catalase is just the right size for a hydrogen peroxide molecule. So the hydrogen peroxide molecule fits exactly into the active site of the catalase and the hydrogen peroxide is split into water and hydrogen, leaving the catalase unaltered ready to accept another hydrogen peroxide molecule. ...read more.

Middle

Concentration of catalase: The higher the concentration of catalase the more hydrogen peroxide that can be broken down at once. This will increase the rate of the test. Concentration of substrate: If you increase the amount of hydrogen peroxide then the time it takes for the catalase to collide with it is decreased so the catalase works more efficiently. This would increase the amount of hydrogen gas so the 10cm� would be collected quicker. This is the variable that I am going to change. I am going to do this by diluting the hydrogen peroxide with water. Variables I will keep constant: I am going to keep concentration of catalase, temperature and surface area the same. I am not going to change these variables in order to keep the test fair otherwise I wouldn't know which variable it was that caused the rate of reaction to change. Apparatus: Boiling tube rack Boiling tube x 6 Hydrogen peroxide Water Ruler Diagram: Method: 1. Set up apparatus as shown in diagram. 2. Place 3cm� of liver puree in each test tube and record the height of froth in a table. ...read more.

Conclusion

When a successful collision occurs a reaction takes place with turns Hydrogen Peroxide into Water and Oxygen. Evaluation: This investigation was a success and easy to perform and the results collected clearly show an increase in height of froth when the concentration of Hydrogen Peroxide is increased. During the experiment I found that measuring the height of froth slightly harder than expected mainly because of the test-tube rack. I found that I had to either remove the test-tube from the rack to measure it, which meant the froth had time to disperse or I had to measure the height a few centimetres away from the test-tube, meaning if I did not look straight on in front of the test-tube and ruler it could give the illusion of being lower or higher froth than it actually was. Another problem is that glass is well known to distort light and therefore any measurements I was taking may not have been accurate because the froth could be at a different height than it appears. There doesn't appear to be an anomalous result on my graph. I know this because my line of best fit roughly goes through all of the points. ...read more.

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