Changes in Temperature affecting Amylase and Starch

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Valentina Zunarelli

IB Biology Practical

Changes in Temperature affecting Amylase and Starch

Aim

         Testing whether a change in temperature will affect the way amylase, an enzyme, acts upon starch (breaking it down), and finding amylase’s optimum temperature.

Apparatus

· Bunsen Burner

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· 10ml measuring cylinder

· Thermometer

· Stop clock

· Iodine solution

· Starch

· Amylase

· Spotting tile

· Pipettes

· Two test tubes

· Tongs

Method

Firstly, fill the spotting tile indents with iodine (about 5 drops) using a pipette. Then, in two test tubes separately, heat 5ml of amylase solution and 10 ml of starch solution starch. Keep heating over a bunsen burner holding the test tubes with tongs until the temperature of the solutions is 30°C, use a thermometer to be accurate. Once the temperature has been reached mix the two solutions together by ...

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