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Chromotography - experiment writeup

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Introduction

Science at work Unit 2: 2.3 Risk Assessment When I will do my experiment I will make sure that I wear my lab coat at all times when doing my experiment to protect my clothes from any spillages. I will also be wearing my goggles to protect my eyes from any of the substances which I will be using. I will also make sure to wear gloves when handling any chemical substances to protect my skin from any of the substances being used, and to prevent any contact being taken place with my skin and any of the chemicals. I will also be careful to prevent any glass breakage taking place. Broken glass can cause open wounds to take place and even create a deep cut. But if any glass does break then I will make sure I keep the area where the breakage has taken place is clear and notify by subject teacher and try and clear up the area carefully. And when I use any electrical equipment I will make sure that my hands are dry and are not wet because electricity and water do not go on well together. I will be using the following chemicals: Copper Salts. ...read more.

Middle

Brass fillings. I will also make sure that none of the fillings get into my eyes as they could severely irritate my eyes by scratching the outer layer of my eye, to prevent this I will be wearing goggles. Plan/Method 1. Complete Risk assessment. 2. Place 30 cm3 of water into a 250 cm3 conical flask, while stirring add 15 cm3 of Nitric acid. 3. Weigh out accurately to two decimals places 1.6 grams of Brass fillings. 4. Add the filling to the mixture in the fume cupboard. 5. Allow the fillings to dissolve over 5 minutes while stirring constantly, during this procedure hazardous fumes can be given off so place the mixture in the fume cupboard to get rid of the poisonous nitrogen dioxide fumes. 6. Remove the solution from the conical flask and add it to another 250 cm3 conical volumetric flask. 7. Then go onto dilute Copper sulphate solution with water to make solution labeled A to F in a table. 8. Switch the colorimeter on and allow it to warm up. 9. Place 700nm filter in the colorimeter and put the meter to zero using a cuvette filled with water. 10. Fill six other solutions labeled A to F and get the absorption values for each of them and record the values. ...read more.

Conclusion

These were all covered in my risk assessment which I already had thought out before I started the experiment which is why I was able to do the experiment without any serious hazards taking place and affecting me in any way. As I had approached each step in my plan I kept referring back to my risk assessment so I had a good idea of what type of risks and hazards were involved. The method which I had used was pretty straight forward and I had practised the experiment using my method before the actual experiment after school in my own time, this helped me understand a bit more about the reason why I am doing this experiment and how I can make my results more efficient. In my method I did not take into account the cuvette, this was a tiny glass piece of instrument which could affect my end results because of little fingerprints being on the out glass surface or small splashes of liquid. This would have affected my results and looking at my results I think I had made I mistake of making the outer surface of the cuvette covered with finger prints which I was able to see or small splash marks from other liquids. ?? ?? ?? ?? ...read more.

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