Core practical(Why does the colour leak out of cooked beetroot?)

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Core Practical

Why does the colour leak out of cooked beetroot?

Introduction:-

Beetroot contains red pigment called betalain, located within the cell vacuole. Normally the pigment cannot pass through membranes but they leak out when the beetroot cooked because the cell membrane begins to breakdown.

Hypothesis:-

My hypothesis is that when temperature increases the proteins in the membrane will be denatured and the pigment will leak out of the cell membranes and will colour the water in the test tubes.

Method:-

Apparatus:- a thin cylinder of beetroot tissues, nine test tubes, beaker ,Bunsen burner, water, thermometer, colorimeter.

Variables:-

* Independent: Time, temperature

* Dependant: