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Designing a data collection strategy The effect of temperature on the rate of respiration of yeast.

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Skill A - Designing a data collection strategy The effect of temperature on the rate of respiration Aim I am going to investigate the question, does a changing temperature have an effect on the rate of respiration in yeast cells? A simple and safe experiment will be conducted by changing the temperature of the yeast and glucose solution, and recording the amount of carbon dioxide produced. Various temperatures will be created in a thermostatically controlled water bath, and a range of temperatures will be used to make the experiment as reliable as possible. Prediction I predict that with every 10�C, the amount of gas which is produced will be doubled. The Q10 of yeast is 2, therefore with every 10�C increase, it will double the rate of reaction, increasing the amount of gas made by 2.1 Q10 is the respiration rate at 10�C/respiration rate at t�C. I believe this will happen because, as the temperature rises there is an increase in kinetic energy of the enzymes in the yeast cells which creates more chances of enzyme substrate complexes being formed, because there are more collisions happening between the enzymes and substrates. This in turn will increase the reaction rate and more carbon dioxide will be produced. So I believe that as temperature increases there will be more gas produced. Each reaction has an optimum temperature where the maximum amount of enzyme substrate complexes will be created, with this experiment I predict it to be around 35�C.2 But eventually it will plateau off, such as at 50�C, once enzyme activity is at its highest and can no longer increase because there is a limited amount of substrates available. ...read more.


I am also going to read the temperature on the water bath to the nearest degree and I will try time the 60 seconds to as accurately as possible to the nearest second. During class experiments I have come to notice that using a thermostatically controlled water bath would be more reliable than using a hot plate to heat the water in which the enzyme substrate complexes will be formed. This is because the water is directly heated in a water bath to the temperature desired, however a hot plate will be heated to the correct temperature, and does not control the temperature of the water directly, adding an extra observation to the investigation. 10 I have chosen to measure volumes of gas produced instead of counting bubbles because many factors can alter the data produced to the true data. For example not all the bubbles may have been counted in replicate as the bubbles are moving fast and can become hard to see or the person carrying the experiment is distracted. Also each bubble may have a different volume of carbon dioxide making this experiment inaccurate when making comparisons. Key factors How it will be regulated/controlled Enzyme type and concentration Yeast will be used in every replicate and each reaction will have 5g of the enzyme. Substrate type and concentration The substrate being used is glucose at a 5.0% concentration. Speed of magnetic stirrer Keeping the speed constant at a medium speed will control the degree of stirring. ...read more.


temperature so the results are as accurate as possible as there will be no reading varied by the glucose starting at a different temperature. This is equilibrating the water bath temperature to the enzyme. Adding buffer solution To make sure that the PH level of the solution is controlled so that the PH is not an effecting factor changing the results. Opening the tap on the burette to release the water until it has reached 0 This is to make the reading easier as the gas amount will be shown straight away without having to take away any values Repeating the experiments at different and same temperatures There are replicates that take place which are done to make the data collected accurate. They are repeated at different temperatures to answer the question if temperature does affect the rate of reaction with yeast cells. Data Processing When processing the data, I will calculate the mean of each temperatures 3 replicate results of which then this figure will be used later to produce a graph showing the overall results. This will provide a more detailed result as I can calculate standard deviation and standard error. During the investigation practical I will be collecting the results on a raw data table, for example the one shown in insert 1. I found an example to base my table on from a website. 13 The independent variable (volume of gas produced every 60 seconds) will be noted down in the corresponding gaps. I will be drawing a line graph, such as insert 2. ...read more.

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