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Diffusion of the beetroot discs dye in different bile salt concentration.

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Analysing evidence and drawing conclusions In this experiment I generated one set of data plus two additional sets were given for the measurement of light absorbance of beetroot dye using the colorimeter. The data are listed below in Table 1. The first column shows the concentration of the bile salt, the next three columns show the absorbance of the three sets of beetroot dye. The last column gives the average absorbance value for each bile salt concentration. Table 1 Concentration of bile salt (g/100cm3) Absorbance (set # 1) Absorbance (set # 2) Absorbance (set # 3) Total Average absorbance (A) 0.0 0.08 0.08 0.09 0.25 0.08 0.1 0.11 0.07 0.04 0.22 0.07 0.2 0.13 0.08 0.06 0.27 0.09 0.3 0.10 0.11 0.17 0.38 0.13 0.4 0.16 0.17 0.20 0.53 0.18 0.5 0.21 0.09 0.21 0.51 0.17 0.6 0.24 0.09 0.27 0.60 0.20 0.7 0.30 0.15 0.31 0.76 0.25 0.8 0.14 0.13 0.32 0.59 0.20 0.9 0.25 0.25 0.36 0.86 0.29 1.0 0.32 0.25 0.41 0.98 0.32 From the above table there is a clear correlation between the concentration and the absorbance of light. As the concentration increases so does the absorbance increase i.e. the concentration is directly proportional to light absorbance. Bile salts cause beetroot cell membranes to become more permeable, therefore allowing the beetroot anthocyanin to diffuse from within the cell to the outside solution. ...read more.


The value of the Rs for 11 pairs of data at 95% probably is 0.61. The value of the correlation coefficient in my experiment is 0.97 which is greater than 0.61, therefore there is a positive correlation between the concentration of bile salt and the diffusion rate of dye from beetroot cells, I can then say my data supports my hypothesis with 95% confidence. Therefore I have rejected the null hypothesis and accepted the working hypothesis. The rest of the 5% was probability due by chance. The meaning of the statistical test signifies to us ,that we are 95 % confident that the bile salts are causing the removal of the protein from the membranes, thus releasing more anthocyanin through the tonoplast. Conclusions Diffusion of the beetroot discs dye in different bile salt concentration. A vacuole is a membrane-bound organelle that usually contains liquid. All cells have vacuoles, but plants cells differ from animal cells in that their vacuoles are very large, permanent, and usually occupy a position fairly near the centre of the cell. The membrane surrounding a plant cell vacuole is often known as the tonoplast. Plants cell vacuoles contain many different substances in water. These include sugars, storage proteins, pigments such as anthocyanin found in a beetroot vacuole and enzymes. Vacuoles have a wide variety of functions. For example the colour of some flower petals are caused by the pigment held inside vacuole in their cell. ...read more.


Collision theory, theory used to predict the rate of chemical reactions particularly for gases. The collision theory is based on the assumption that for reaction to occur it is necessary for the reacting species (atoms or molecules) to come together or collide with one another. Not all collisions however, bring about chemical change. A collision will be affective in the producing chemical change only if the species brought together possess a certain minimum value of internal energy, equal to the activation energy of the reaction. Furthermore, the colliding species must be oriented in a manner favourable to the necessary rearrangements of atoms and electrons. Thus according to the collision theory, the rate at which a chemical reaction proceeds is equal to the frequency of collision can be calculated with some degree of accuracy only gases (by application of the kinetic theory), the application of the collision theory is limited to gas phase reaction. This theory shows why at higher concentrations of bile salts, the absorption is higher. It is because at higher concentration of bile salts molecules collide with membrane more frequently therefore more damages is caused to the and more anthocyanin is released, making absorption readings higher. Amylase blinds with ester linkage at a special site known as the active site, which has a specific shape that will only fit that portion of the molecule-it is specialised. This is known as the lock and key hypothesis. Bile salt has no effect on the cell wall, which is made of cellulose, because it is full permeable to pigment and bile salt. ...read more.

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