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Digestion Of Gelatin On Exposed Photographic Film.

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Digestion Of Gelatin On Exposed Photographic Film Introduction Enzymes are biological catalysts, that speed up the rate of a chemical reaction at low temperatures (Body temperature) without being changed or used up themselves. How Enzymes Work Enzymes are proteins that behave as catalysts. They speed up biological reactions but remain unchanged by the reaction. The enzyme is shown in blue and the molecule to be broken up (the substrate molecule) is show in red. The enzyme contains an active site. Part of the substrate molecules fits into this site like a key fitting into a lock. This allows bonds in the molecules to break. The temperature affects the rate of reaction, as the temperature increases so does the rate of reaction this is because the substrate molecules are given extra kinetic energy from the source of heat, which enables them to move round faster which consequently means that the chances of the substrate molecules colliding with the enzyme that it breaks it down is much likely then before. ...read more.


Apparatus * Thermostatically water bath * 5 Boiling tubes * 5 Thermometers * 5 Stop watches * 1% Trypsin * Measuring cylinder * 5 Splints * 5 Photographic Films * 5 Test tube racks We used thermostatically water bath so we could get the exact temperature for our experiment, the thermostatically water bath is very accurate. We used 5 boiling tubes, 5 thermometers, 5 stopwatches, 5 splints, 5 photographic films and 5 test tube racks so we could do 5 experiments at the same time but at different temperatures. We used the measuring cylinder so we could measure out 10cm3 of the enzyme trypsin for every experiment; the measuring cylinder is suitable for this because it is accurate and would help us to keep our experiment fair. Method First of all we measure out 20cm3 of 1% trypsin using a measuring cylinder. Then we pour the trypsin into a boiling tube. We place a cork on top of the boiling tube (the cork has a whole in it) ...read more.


As you can see the higher the temperature the faster it is for the Trypsin to react with the photographic film. I would have repeated each experiment 3 times to get a good result, but I could not as we did not have enough Trypsin. Evaluation There appears to be no clear anomalous results. As my method and plan was to make the experiment a fair test and if I had made sure that the results were exactly accurate, the results should have been near perfect. But because I could not ensure exact measurement was used, this could be a possible reason for the slight inaccuracy in the results. My prediction was right, I said that the enzyme will work good around 40 degrees and the enzyme denatured at 45 degrees. If I had the chance I would redo the entire experiment, as I could not do it properly because we did not have enough Trypsin. I would also repeat my experiment more then 5 times just to make sure I get nice results. My results are not very reliable as they are not accurate. I think my method was suitable for this experiment it was very simple. ...read more.

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