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Digestion report

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Effect of temperature on digestion due to Amylase Aim: Test the effect of temperature on the digestion of starch into maltose by amylase. Hypothesis: there will be the highest amount of maltose between 30�C and 40�C. Thus, the amylase will digest the most starch between 30�C and 40�C. Materials: - 1) Starch solution - 2) Amylase - 3) Test tubes (A, B, C) - 4) Bunsen burner - 5) Tripod stand - 6) Iodine - 7) Gauze - 8) Plastic dropper - 9) Beaker - 10) Water - 11) Petri dish (A, B, C) Procedure: 1. fill a beaker until it is about half-way filled with water 2. then suspend it over the Bunsen burner, using the tripod stand and the gauze 3. ...read more.


Iodine was added to determine the levels of starch in the solution. The results are shown in the table below: Test Tube Temperature (�C) Color A 35 Very light blue-black B 40 Dark blue-black C 50 Very dark, almost black Data Analysis: Working through this experiment I have analyzed the effect temperature has on digestion due to the amylase. With test tube A, 35�C, the solution was a light blue-black. As we increased the temperature, first to 40�C and later to 50�C, the solution got a bit darker. At 50�C, the solution was bordering on almost being completely black. From the results, I made the following analysis. Amylase reacts with starch to produce maltose. This means that, the more amylase there is the more starch will be converted into maltose. ...read more.


Primarily, are the chosen temperatures that I drew my results from only show one side of the data. The temperatures I chose were too high, and didn't give me enough of an indication as to where the optimum temperature was. Ideally, if I were to do this experiment again, I would choose lower temperature values - 20�C, 30�C and 40�C. This way I could amylase my results better. Another error in this experiment was that the amount of amylase and starch we mixed might have been inconsistent. The dropper might not have given us the precision we would like when measuring the amount of starch and amylase to add to the solution. Next time, I will pour the starch and amylase into separate measuring cylinders, before using them in the experiment. Personally, this experiment was very interesting and helped me understand better the lesson we are learning. ?? ?? ?? ?? Simon coste October 18 ...read more.

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