Does caffeine affect heart rate?

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Laura Chipperfield

CORE PRACTICAL

Does caffeine affect heart rate?

Method

  • Use a pipette to transfer a daphnia to a cavity slide. Remove the water around the daphnia using filter paper. Add one or two drops of distilled water
  • Look at the daphnia through a microscope. Use a stopwatch to record the number of heartbeats per minute. Record the heart rate at intervals of two minutes over a 10-minute period.
  • Repeat the procedure using other daphnia in fresh slide and cover slips. Replace the water with caffeine solution. Repeat the procedure using several different concentrations of caffeine.

Results

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Why does colour leak out of cooked beetroot?

Method

  • Cut sections from a single beetroot. Cut eight, 1cm length slices from these sections.
  • Place the slices in a beaker of distilled water. Leave overnight to wash away excess dye.
  • Next day, place eight labelled boiling tubes each containing 5cm3 distilled water into a water baths at 0, 10,20,30,40,50,60 and 70 degrees. Leave for 5 minutes until the water reaches the required temperature. Place one of the beetroot sections into ...

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