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Enzyme Concentration

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Introduction

Enzyme concentration Aim In this experiment, the purpose is to investigate how enzyme concentration can affect the initial rate of reaction. By doing this I will be able to develop experimental and investigate skills, including risk assessment. Hypothesis The effect of reducing the concentration of the protease enzyme on the rate of breakdown of the protein found in the milk powder is that the enzymes will run out of energy which will mean less milk powder will be broken down as they have smaller energy over the time. ...read more.

Middle

Procedure * Pour 2cm? of protein solution into a cuvette using a pipette. * Then pour 2cm? of the protease solution in to a cuvette using another pipette. Mix carefully and quickly put into the colorimeter and start the stop clock. * Measure the absorbance every 30 seconds for 5 minutes or in till there is a little change in the reaction and record the result. * Get rid of the mixture and rinse the cuvette with distilled water. ...read more.

Conclusion

That is why the trend is going downwards because they are losing their energy. Conclusion So overall the enzymes will get used up more and more as the seconds increase which means less of the milk product is being broken-down because energy is getting lost as it is being used up. Evaluation The results are reliable but we don't know what type of milk it is, so in the future to make it even more reliable we should test different types of milk. ...read more.

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