Enzyme Concentration

This essay hasn't yet been marked by one of our teachers

You can view 21 essays on Exchange, Transport & Reproduction that have been Marked by Teachers

The first 200 words of this essay...

Enzyme concentration

Aim

In this experiment, the purpose is to investigate how enzyme concentration can affect the initial rate of reaction. By doing this I will be able to develop experimental and investigate skills, including risk assessment.

Hypothesis

The effect of reducing the concentration of the protease enzyme on the rate of breakdown of the protein found in the milk powder is that the enzymes will run out of energy which will mean less milk powder will be broken down as they have smaller energy over the time.

Equipment

* Milk powder solution

* Test tubes

* Test tube holder

* Stop clock

* Standard protease solution 1%

* 5cm? pipettes

* Glassware for diluting enzymes

Safety

All enzymes are potential allergens. Handle with care to minimize skin contact and inhalation. Also wear safety gear such as goggles and aprons.

Procedure

* Pour 2cm? of protein solution into a cuvette using a pipette.

* Then pour 2cm? of the protease solution in to a cuvette using another pipette. Mix carefully and quickly put into the colorimeter