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Enzyme Concentration on Enzyme Activity

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Introduction

The Effect of Enzyme Concentration on Enzyme Activity. Aim- I am going to find out how the times of the protein which can be separated from photographic film by an enzyme (Savinase) are altered, simply by changing the concentration of Savinase. Introduction- Cystic Fibrosis sufferers usually have poor digestion, because of the fact that the pancreatic duct becomes blocked, which limits the enzymes released into the small intestine. I am Hypothesis- I think that the more enzymes that are present in a solution/area, that the faster the reaction will take place, up to a certain point. The reaction will keep on increasing in speed due to the fact that the more enzymes that are there, the more particles can be reacted and thus the reaction will happen faster. If there more enzymes than particles to be reacted, then the reaction will not go any faster, since the extra enzymes will not do react. The more enzymes there are, the faster the reaction goes (up to a point), but this does not mean the enzymes are any more or less effective, each enzymes does it's job just as quickly, there are just more enzymes to go around. ...read more.

Middle

Continue this, finishing at test tubes labelled '5', where 5ml of Savinase is added, with no buffer. Cut the photographic film into twelve equally sized strips, short and narrow enough so that they will be submerged in mixture and that they will fit in the test tube. Ideally, six test tubes should in one test tube rack, the other six (a repeat test) in another. Put both of the racks into the 40�C water bath (do not submerge the test tubes, just bath them over the level of mixture in the tubes) and time five minutes on the stop clock, so the mixtures in the test tubes should rise in temperature (to 40�C). After the five minutes have passed, add one strip of film into each test tube, try to do this quickly, so that each strip has been in just as long as each other. Reset and start the stop clock again, and go back to the test tubes. Gently agitate each test tube (can be easier with a partner, so each test tube is tendered to at the same time) ...read more.

Conclusion

be agitated equally, since if one is agitated a lot more than another, the protein may come apart earlier than it should, and one may have separated slower, due to it not being agitated enough. Another thing I would change is the method of inserting the film strips, since they are inserted at slightly different times, and this will lead to error. To fix this I would insert each one at ten or thirty second intervals, and then work out the difference in time relative to the others. Conclusion- From this experiment I can deduce that the concentration of an enzyme does effect the time the reaction takes, and the lower the concentration, the slower the reaction takes to complete. The higher the concentration, the faster the reaction takes to complete, but up to a point (where there are the same amount of particles to react and enzymes) the reaction will not take any less time, since the enzymes will react with one particle each, and this is the fastest situation, the extra amount of enzymes will be useless. ?? ?? ?? ?? ...read more.

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