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Enzyme Concentration Practical

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Enzyme concentration experiment In this experiment I had to investigate how enzyme concentration affects the rate of reaction. My hypothesis is that the higher the concentration of enzyme solution the faster the reaction will take place. If we have more enzymes in a solution then we have a higher chance of them colliding with the gelatine jelly molecules and break them down. If we have a lower concentration of enzymes then they will collide less often and have a slower rate of reaction. 5% enzyme 1% enzyme solution solution For this experiment I have used the following equipment: * 75g of gelatine jelly * 5ml of enzyme solution * 5ml of water * 5ml of ph Buffer * Syringes * Beakers * Funnels * Water bath with water at 37�C * Spoon * Scale Method I placed 70g of gelatine jelly into a beaker - 75g of jelly is more than enough and I will be use this amount all the time, it would take longer and it would ...read more.


Fair testing In order for the experiment to be fair I need to be careful with the following factors which could affect my results: * I must use the same amount of gelatine jelly each time otherwise my results will not be valid, the jelly also being my dependent variable * I must make sure the jelly is the same size pieces otherwise is they're different sizes it could affect the rate of reaction. * I must use the same volume of enzyme solution each time, * I must use the same filter funnel * I must leave the solution in the water bath for the same length of time each time because if I leave them for too much or too less then the enzymes will either have digested too much or not had enough time to react. * I must have the water bath set at 37�C each time if it goes higher then the enzymes will begin to denature and stop working properly. ...read more.


Sources of error The gelatine wasn't the same size chunks and had different surface areas, a larger surface area increasing the reaction and a smaller surface area would slow down the reaction. The effect that this had on the reaction was that the rate of reaction had been changed. A mold could have been used to measure each chunk. The amount of time the enzymes were left in the hot water might have not been the same every time. If it was left more the enzymes would have digested more and thus changing the rate of reaction. The equipment used to measure, the syringes we had to measure the low volumes weren't as accurate as it could be to measure the small volume. Evaluation I believe that my experiment was successful in proving my original hypothesis. I think that the number of repeat results taken shows that the data obtained was reliable, since three repeat experiments is enough to prove the basic idea behind a hypothesis. ?? ?? ?? ?? ...read more.

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