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Enzymes - In this experiment I am trying to find the best conditions for amylase to digest starch.

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Introduction

Introduction: Enzymes help to turn a substrate into the product. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific substrate. This is called the lock and key theory. In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one helps digestion the fastest. This will show which PH enzymes will work best in. I will put 3cm� of 1% amylase solution, 1cm� of 0.5% starch solution and 1cm� of the different acid or alkali I am testing to each test tube. I will then heat them in a water bath to 40�. I will then every 2 minutes test the solution for starch using iodine solution. I already know that temperature adds kinetic energy to the process so will break down starch faster because the molecules are moving faster and will speed up the process. ...read more.

Middle

measuring my solutions Starch - Used as part of my solution for testing enzyme Amylase - Used as part of my solution for testing enzyme Buffer - PH6, PH7, PH8, PH9 acids and alkaline Dimple Tray - This is where I will test my solution for starch Iodine - Used to test my solution for starch Preliminary Tests: I will use these tests to see whether I need to change my amounts of starch (1cm3 ), amylase (3cm3 ) and buffer (1cm3) or the times when I measure the solution. I have set the temperature to 300C. Time(mins) Is Starch Present 1 Yes 2 Yes 3 Yes 4 Yes 5 Yes 6 Yes 7 Yes 8 Yes 9 Yes 10 Yes 11 No The experiment was taking too long so we changed the time when we measured the solution to 2 minutes rather than 1 and increased the temperature to 400C. Test: We first tested PH6: Time(mins) ...read more.

Conclusion

because I could only the equipment I had available in school which although it gave me generally good results could still have been more accurate. As I predicted I saw the pattern in my results that the higher the concentration the faster the digestion will take place until I got to PH 9 which took longer than PH7 and PH8, this is probably when the concentration was too high for the molecules and they started to denature. Evaluation: I think my experiments worked well and produced some clear and accurate results that almost matched what I had predicted before the experiments took place. I think I could have improved my investigation by testing each concentration once more to improve my accuracy of results and also test PH10 alkali to see whether the enzyme continued to denature at the rate that PH9 did to again improve the accuracy of my results. I think I could have also used more accurate equipment to improve my results but I still generally got a good amount of accuracy. Luke Gilks ...read more.

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