• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzymes - In this experiment I am trying to find the best conditions for amylase to digest starch.

Extracts from this document...


Introduction: Enzymes help to turn a substrate into the product. The enzyme has an active site that helps it to recognise its substrate in a very specific way. Just like a key only fits into a specific lock, each enzyme has its own specific substrate. This is called the lock and key theory. In this experiment I am trying to find the best conditions for amylase to digest starch. I am going to vary the PH from PH6 to PH9 to see which one helps digestion the fastest. This will show which PH enzymes will work best in. I will put 3cm� of 1% amylase solution, 1cm� of 0.5% starch solution and 1cm� of the different acid or alkali I am testing to each test tube. I will then heat them in a water bath to 40�. I will then every 2 minutes test the solution for starch using iodine solution. I already know that temperature adds kinetic energy to the process so will break down starch faster because the molecules are moving faster and will speed up the process. ...read more.


measuring my solutions Starch - Used as part of my solution for testing enzyme Amylase - Used as part of my solution for testing enzyme Buffer - PH6, PH7, PH8, PH9 acids and alkaline Dimple Tray - This is where I will test my solution for starch Iodine - Used to test my solution for starch Preliminary Tests: I will use these tests to see whether I need to change my amounts of starch (1cm3 ), amylase (3cm3 ) and buffer (1cm3) or the times when I measure the solution. I have set the temperature to 300C. Time(mins) Is Starch Present 1 Yes 2 Yes 3 Yes 4 Yes 5 Yes 6 Yes 7 Yes 8 Yes 9 Yes 10 Yes 11 No The experiment was taking too long so we changed the time when we measured the solution to 2 minutes rather than 1 and increased the temperature to 400C. Test: We first tested PH6: Time(mins) ...read more.


because I could only the equipment I had available in school which although it gave me generally good results could still have been more accurate. As I predicted I saw the pattern in my results that the higher the concentration the faster the digestion will take place until I got to PH 9 which took longer than PH7 and PH8, this is probably when the concentration was too high for the molecules and they started to denature. Evaluation: I think my experiments worked well and produced some clear and accurate results that almost matched what I had predicted before the experiments took place. I think I could have improved my investigation by testing each concentration once more to improve my accuracy of results and also test PH10 alkali to see whether the enzyme continued to denature at the rate that PH9 did to again improve the accuracy of my results. I think I could have also used more accurate equipment to improve my results but I still generally got a good amount of accuracy. Luke Gilks ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    Time taken to drop 1.5 arbitrary units/s RANK (y) D (x-y) D2 1 0 2 160 1 1 1 2 0 2 190 3 -1 1 3 0 2 182 2 0 0 4 0.1 5 422 4 1 1 5 0.1 5 452 5 0 0 6 0.1 5

  2. How the concentration of amylase effects the digestion of the starch.

    my prediction-'as the concentration increases the time taken for the amylase to break down the starch decreases' EVALUATING EVIDENCE Evaluating my investigation I have found and proved that my prediction was correct. The experiment with 100% amylase broke down the starch in the fastest time.

  1. Amylase is an enzyme that helps to digest starch into maltose

    Due to technical limitations, I will conduct this experiment only at the following temperatures: 10�C, 20�C, 30�C, 40�C, and 50�C. Method In this experiment I will measure the time taken for starch to be digested with the aid of amylase, at different temperatures.

  2. Catalyse Investigation

    When the potassium permanganate was added the solution immediately turned purple. This colour gradually faded until it became completely decolourised. In the table, for the temperature 100�, I have used the symbol for infinity for the time reading, since at this temperature, the potassium permanaganate did not decolourise.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work