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Enzymes Investigation

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Steven Vickery 11E SC1 Biology Practical Investigation Hypothesis; Enzymes are biological catalysts. They speed up chemical reactions, which take place inside living things. Molecules are constantly moving about and bumping into each other. Now when a substrate molecule bumps into a molecule of the right enzyme, it fits into a depression on the surface of the enzyme molecule. This depression is called active site. The reaction then takes place and the molecules of product leave the active site, freeing it for another substrate molecule. In this investigation we are going to see what the atmosphere and the environment have on the breaking down of proteins via a protein-digesting enzyme. Safety Precautions; To make sure this experiment is safe, I have decided that the water bath temperature range during the experiment will not cause physical damage (e.g. scolding). The protein digesting enzyme bottle will also be clearly marked as,"Harmful if swallowed". Steps will also be taken so passers by will not accidentally drink the enzyme. I shall do this by labelling the substances I obtain. Fair Test; Before I proceed with the Experiment, I have taken into account the following; 1. It is important that all apparatus for this experiment is clean and will rinsed prior to each experiment. 2. All chemicals used will be checked to make sure that no obvious signs of contamination have occurred. ...read more.


To obtain the results I used precise and skilful equipment, to get the most accurate results. The most important facility was an electronic timer, which was accurate to the ms. Steven Vickery 11E Analysing and Concluding Explanation There is no data for 20 to 25�C all the way through the results and replicates, because I suspect the enzyme was either working really slowly, or the enzymes had died due to the cool conditions. I think that the results obtained in the first two sets of results are more similar than the third set of results, but if I had more time, I would have pursued this matter. I would have done this by making another set of results. Then I could see if the third set of results had any comparisons with the first and second set of results. Below is a graph representing the results from the first set of results. Steven Vickery 11E This graph shows a reasonably smooth curve, which gives a good indication in what sort of temperature range, it will denature the enzyme. Below I have constructed a graph showing both the first set of results and the second set of results. These results have quite a similar trend, which is very obvious. However, I maybe able to draw some comparisons if I construct the same table but with all three sets of results so I will be to outline differences and similarities. ...read more.


My results could have been more reliable if I washed the test tube with water and not drying it, which was maybe one of the reasons why that the third set of results was anomalous. Another problem was during the experiment when a test on the broken down powdered milk had to be compared with the control. Sometimes our analysis was inaccurate when it may of needed more powdered milk to be broken down. I think I should have washed all of the equipment used, (apart from the stop clock) because the other equipment may also have been contaminated from previous experiments. I think the experiment results were sufficient to reach a basic conclusion, but more data would have made the averages more accurate. I think the anomalous results throughout the experiment were largely as a result of a few points. * Inaccurate timing * Contaminated equipment * Contaminated solutions These were potentially a big set back but if I was given extra time to collect additional data, my results would be accurate and more accurate conclusions could be made. I think I could have improved the experiment by using a larger range of enzymes, so an overall comparison could be made. Different dairy products could be used so we could find out rate of reaction on certain foods. A more accurate scale of temperatures could be used to see what degree the perfect temperature is. I would have carried these experiments out if I was given extra time. ...read more.

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