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Enzymes Investigation

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Biology Coursework Enzymes Enzymes are a substance that acts as a catalyst in living organisms. Enzymes are found in the mouth, stomach and small intestine of mammals. There are many types of enzymes such as lipase and protease. Each different type of enzyme has its different type of food to speed up the reaction of its breakdown. Even after the enzyme has done its job, its structure still remains unchanged. Humans need enzymes to break down food into small molecules, small enough to be absorbed in the small intestine. Here are two examples of the substrate (food molecule), enzyme and its product. Amylase Starch --> Maltose Starch phosphorylase Glucose --> Starch Enzymes work like a lock and key, the key fits in and out but the lock remains the same. Substrate--> Enzyme--> Product--> All enzymes are proteins, and their molecule is a very precise three-dimensional shape containing an active site. The active site is the most important part of the enzyme, it is where the substrate fits in to the enzyme and is broken down. It breaks the substrate molecule down by doing something called 'tweaking' that pulls the substrate out of shape breaking it apart. ...read more.


If the enzyme is in a very alkali or acid environment it will not work as well. Method For this experiment we will have to have an enzyme and substrate. We will also have to have an acid. I have chosen these three chemicals to be used in our experiment- - Enzymes = Liquidised Potato (catalase) - Substrate = Hydrogen peroxide - Acid = Hydrochloric acid To make different Ph's I will dilute the hydrochloric acid according the Ph I need. Our apparatus are- - Test tube - Bung - Delivery tube - Measuring cylinder - Bowl filled with water - Litmus paper I will do the following to keep the experiment safe and fair... - Wear goggles to stop the acid damaging my eyes. - Tuck my tie in my shirt. - Keep my shirt arms rolled up to stop them dragging across the apparatus. - Keep my book away from the experiment as not to spill water on my results table. - Keep the same amount of water on the bowl & and the same tempreture. - Keep the same amount of potato in the test tube - Keep the same amount of substrate in the test tube - Keep the same lenth delivery tube if repeating results - Carry the experiment out in the same room at the same tempreture. ...read more.


by twisting the molecule and breaking the molecule apart it would divide the molecule making two instead of one molecule of hydrogen peroxide. As this reaction takes place it creates oxygen so we know from the more oxygen made the more successful collisions have been made. I did have a couple of wrong results that took place in my 1st experiment but I redid them and found the correct result that fitted correctly into my graph. Conclusion The experiment went well, and it did what I expected; I did get some false results in my experiment but I managed to redo them. The initial result could have happened from a bubble of oxygen missing the measuring cylinder, or the cylinder being lifted slightly too much and air getting into the cylinder. I could have overcome this problem by using a gas syringe that would probably have been more accurate and less easy for gas to escape from. My results can be trusted to be correct as I did do them in safe and fair conditions and only a couple of results go stray of the pattern of the others. I have found that the enzyme catalase works to its maximum at Ph 7 which is a neutral Ph. ...read more.

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