Examine how the concentration of the temperature Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.

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a) Aim:

 How does yeast action in different temperature? How much oxygen made? From this to know where the optimum temperature is. To investigate the effect of temperature on the enzyme catalase.

This is an experiment to examine how the concentration of the temperature Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.

b) Background Information:

i) Yeast developed into history:

The name comes from the Greek meaning ‘in leaven’ or yeast. Egyptian has long known use yeast very much to make wine. Making the principle of wine is using the sugar in grain or fruit, train yeast to make it grow up, in the thing that produces, having a share alcohol, have a little partial fragrant material additionally, has caused the wine kind of flavors that attracts people.

The ancient’s people do not know the role of yeast. Therefore, they feel that make wine is very having magical conversion strength. It can make grain or fruit to the wine drunk. Now, we know that yeast is for initial matrix with carbohydrate, untie glycolysis to make alcohol.

We go to the beginning of 20 century; we still do not understand this kind of nature change strength is developing beginning from organic chemistry. Gradually, we just know is the certain of material in yeast bacterium, compose the conversion reaction of carbohydrate, analyses to get is a kind of protein.

In 1926 Sumner first time to found a kind of enzyme called unease crystallization. This is one of significant discovery for biological chemistry. Because have magical catalysis ability yeast, unexpectedly can picture generally on test tube and beaker to be crystallization. Yeast is physiological role for cell nearly omnipresent. Long time ago the human did not know how effect us, but enzyme can make partial relation. For example, used to in pesticide for cats and dogs is a kind of yeast inhibitor (acetyl cholinesterase inhibitor). If human or animal suck it into body. It can make people or animal become very irascible.

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ii) Temperature:

 As temperature increases, molecules move faster (kinetic theory). In an enzyme catalysed reaction, such as the decomposition of hydrogen peroxide, this increases the rate at which the enzyme and substrate molecules meet and therefore the rate at which the products are formed. As the temperature continues to rise, however, the hydrogen and ionic bonds, which hold the enzyme molecules in shape, are broken. If the molecular structure is disrupted, the enzyme ceases to function as the active site no longer accommodates the substrate. The enzyme is denatured.

An enzyme is ...

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