Experiment To Investigate the Plasmolysis Rate and Concentration of Sucrose Solution In the Onion Cell
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Experiment to investigate the plasmolysis rate and concentration of sucrose solution in the onion cell Neena Kim SFC1A Date of the experiment : 1st September 2001 Objective In the onion cell, there is cell sap which has a certain concentration of materials. Depending the concentration of the outside of the cell, the cell can be plasmolysed at the different rate by osmosis.
fine forceps paster pipettes slide and cover slips microscope graph paper lazor blade tile procedure label six % of plasmolysed cells in the onion concentration of sucrose solution 1st 2nd 3rd average 0.2M 0.0 3.6 1.7 1.77 0.3M 15.8 22.0 22.1 19.97 0.35M 46.8 56.3 38.5 47.18 0.4M 69.3 54.5 57.3 60.40 0.5M 94.6 91.2 89.2 91.70 1.0M 98.7 95.1 96.0 96.67 Table of the percentage of the plasmolysed cells in the onion and the concentration of sucrose solution used Limitation factors 1.
2. The used part of onion cell is not even and identical. The concentration of cell sap is different from place to place so that the degree of plasmolysis can be different. 3. The time may affect the result. Although the osmosis will stop when it reaches the equilibrium, we do not know when the equilibrium point is. Therefore, we might take the onion piece out too earlier so that the plasmolysis would not be fully accomplished. 4. the other component within the cell sap may give the unwanted error. 5. 6. 7. 8.
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