Experiment To Investigate the Plasmolysis Rate and Concentration of Sucrose Solution In the Onion Cell

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Experiment to investigate the plasmolysis rate and concentration of sucrose solution in the onion cell

Neena Kim SFC1A

Date of the experiment : 1st September 2001

Objective

In the onion cell, there is cell sap which has a certain concentration of materials. Depending the concentration of the outside of the cell, the cell can be plasmolysed at the different rate by osmosis.

Hypothesis

The cells will be plasmolysed more as the concentration increases. And incipient plasmolysis is the point when 50% of the cells are plasmolysed.

Around 0.35M point, the rate of plasmolysis is the greatest.

Apparatus

Onion bulb

6 petri dishes

6 test tubes

test tube rack

wax pencil

six 10cm graduated pipettes

six beakers

fine brush

distilled water

1M sucrose solution

fine forceps

paster pipettes

slide and cover slips

microscope

graph paper

lazor blade

tile

procedure

label six

% of plasmolysed cells in the onion

concentration of sucrose solution

1st

2nd

3rd

average

0.2M

0.0

3.6