• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to show the effect of Temperature on membrane permeability In beetroot

Extracts from this document...

Introduction

Experiment to show the effect of Temperature on membrane permeability In beetroot Joseph Colledge Introduction Plants are unique among the eukaryotic organisms whose cells have membrane enclosed nuclei and organelles, because they can manufacture their own food. Chlorophyll, which gives plants their green colour, enables them to use sunlight to convert water and carbon dioxide into sugars and carbohydrates, chemicals the cell uses for fuel. Like the fungi, another kingdom of eukaryotes, plant cells have retained the protective cell wall structure of their prokaryotic ancestors. The basic plant cell shares a similar construction with the typical eukaryote cell, but does not have centrioles, lysosomes, intermediate filaments, cilia, or flagella, as does the animal cell. (Reed 1998) Plant cells do, however, have a number of other specialized structures, including a rigid cell wall, central vacuole, plasmodesmata, and chloroplasts. In prokaryotes and plants, the plasma membrane is an inner layer of protection since a rigid cell wall forms the outside boundary for their cells. The cell wall has pores that allow materials to enter and leave the cell, but they are not very selective about what passes through. The plasma membrane, which lines the cell wall, provides the final filter between the cell interior and the environment. Lipids and proteins are the staple ingredients of membranes, and, the most abundant lipids in membranes are phospholipids. ...read more.

Middle

Method Apparatus Test tubes Test tube rack Cork borer Tile Scalpel Small beaker Graduated beaker Large beaker Thermometers Water baths 1. Use a cork borer to cut cylinders of fresh beetroot tissue. Then place onto a tile and cut into 60, 2mm wide discs. 2. Place all discs in a small beaker and wash under a running tap for at least 5 minutes until no more pigment is released from the damaged cells. 3. Label test tubes 30�C, 40�C, 50�C, 60�C, 70�C and 80�C 4. Use a graduated pipette to add 20cm� cold water to each of the test tubes. 5. Add labelled test tubes to water baths using a large beaker, tripod and gauze and for any temperatures which aren't provided use a Bunsen burner. 6. Add 10 discs to the test tube and leave and leave in the water bath for 10 minutes. You should see pigment leave the tissue. 7. Remove the test tube from the water bath and carefully decant the liquid into a fresh test tube, leaving the discs behind. 8. Repeat the procedure for the other tubes. Shake the tubes, hold to the light and compare the colour of the liquids in each. 9. With a blue filtered colorimeter place a selection of each test tube in turn and compare the colours of the liquids. ...read more.

Conclusion

There might be a higher controlled permeability for such compounds that are supposed to enter through the membrane but it will not break down, releasing the red beet colour. When the temperature goes beyond these limits water expands, putting pressure on the membranes from within. The lipid part of the membrane liquefies, making it more prone to leakage. The proteins that span the membrane fall apart, creating holes in the fabric. All this combined will allow compounds to exit the cell. The reason this is possible is physics. Higher temperature makes all molecules shake and vibrate more. The faster movement disrupts any ordered structure there might have been, eventually destroying the structure altogether. Another reason for release of the pigment is due to basic osmosis, in that the interior of the cell has a much lower water potential than the outside of the cell so that when there is an increase in the temperature it in turn causes the water to go from the higher concentration to the lower concentration (a typical gradient) The causes the release of the pigment as the walls are literally broken down due to pressures put against them and thereby causing the pigment to be diffused into the surrounding water. To conclude, the hypothesis stated in the introduction did indeed concur with the results found, in that an increase in temperature increased the amount of pigment released. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Beetroot Experiment. The purpose of this experiment is to determine the effect of temperature ...

    4 star(s)

    Variables Dependent variable = temperature. The purpose of this study is to investigate the effect of temperature on the plasma membrane. In order to carry out this investigation, the experiment will be repeated at several temperatures to discover at which intervals the plasma membrane begins to break down.

  2. Marked by a teacher

    Investigating the effect of temperature on the movement of pigment through beetroot cell membranes.

    4 star(s)

    EVALUATION- I feel my results are reliable enough to make a rough analysis from but there are parts of my method that should be improved before carrying out the actual experiment. * A wider range of temperatures is required in order to gain more results so a more detailed analysis can be carried out.

  1. Marked by a teacher

    The Effect of Ethanol Concentration on the Permeability of Beetroot Cell Membranes to Betalain

    3 star(s)

    with 0% ethanol solution, place in colorimeter in exact position, put the cover on top and read the dial, record result to the nearest 1%, pour the liquid back in the boiling tube and clean and dry the tube thoroughly as other solutions may affect the following result.

  2. Marked by a teacher

    AN INVESTIGATION INTO HOW TEMPERATURE AFFECTS THE PERMEABILITY MEMBRANE OF A BEETROOT.

    3 star(s)

    This happens because higher temperature makes the beetroot's molecules to shake and vibrate more. The faster movement disrupts any ordered structure that might have been in the membrane, eventually destroying the beetroot's membrane structure altogether. At also at high temperature, the betalain pigment of beet root cells is normally sequestered

  1. Marked by a teacher

    The effect of temperature on the permeability of beetroot membrane

    3 star(s)

    At higher temperatures the cell membrane will be broken down more so there will be a greater release of the betalain pigment. * Predict the result of a test of the hypothesis to indicate the direction of the link As the temperature increases the phospholipid bilayer and the proteins which make up the cell membrane become damaged.

  2. Marked by a teacher

    An investigation to examine the effects of temperature on membrane stability in beetroot, by ...

    3 star(s)

    The use of these transport proteins means that the type of diffusion that is occurring here is facilitated diffusion; this means that something is helping the molecules to diffuse through. However, no energy is required in diffusion. Proteins are built up from smaller subunits called amino acids.

  1. Marked by a teacher

    Effect of different temperatures on the permeability of beetroot cell membrane.

    3 star(s)

    My reason for this is a protein is affected by temperature, when temperature is increased the 3D shape of the protein is changed causing it to become denatured. Proteins are hydrophilic channels for ions and polar molecules, also allowing certain substances enter and leave the membrane and if the proteins

  2. An experiment to find of the isotonic point of root vegetables cells in contents ...

    1.33 0.33 -0.67 -1.33 -2.66 Percentage Change (%) 4.43 1.10 -2.23 -4.44 -8.88 ANALYSIS The pattern my results and graph have shown are that the higher the concentration of the sucrose solution the more likely it is that the root vegetable cylinders mass will decrease as all my line of

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work