I used a programme on the computer to carry out my experiment for maximum accuracy and controlled conditions, but the apparatus that would be needed to carry out the experiment would be a thermometer, a stop clock, a water bath, a
, a and a once the apparatus is set up to do the experiment you have to have the water bath at a certain temperature to get your first result, the result being the amount of co2 given off from the yeast respiration, you should also take down the time and temperature to help when doing a graph and for finding out the effect the temperature actually had on the speed of the experiment. You should repeat this over equal intervals of time.
I predict that for my experiment the amount of co2 produced will increase as the temperature does this is because of the collision theory, this being that the higher the temperature the more molecules have energy to move around the beaker, and the more they move around and the faster they do it the more collisions will take place ending up in more reactions taking place, but once the temperature reaches a certain point the amount of co2 produced will slow down because the enzymes will denature meaning the substrate will no longer fit the enzyme.
Whilst analysing my results I realised that my prediction was quite accurate as the temperature increased by ten degrees each time up until forty so did the amount of carbon dioxide and although I did not predict an exact temperature the amount of carbon dioxide did slow down when the temperature increased to a certain point, in this case it was fifty degrees.
In Conclusion the temperature does increase the amount of carbon dioxide produced in yeast respiration, and this is due to the collision theory as the temperature increased so did the number of collisions in the time period we were testing and as a result of this more reactions took place, the reasoning behind the slowing of the reaction after forty degrees is that the enzymes did de-nature and the substrate did no longer fit and so slowing the reaction.
I feel that my results are highly reliable as the test conditions were perfect due to the programme used on the computer, I was happy with my method as doing on the computer made testing only one variable at any one time very easy. I think that my results are accurate enough to back up my conclusion although I could repeat the test more times to get an even more accurate average but I feel that 3 times is sufficient enough for my conclusion to be backed up with.
