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Find out how Copper Sulphate affects the activity of Catalase.

Extracts from this document...

Introduction

Enzymes Investigation Aim: To find out how Copper Sulphate affects the activity of Catalase. Hypothesis: My hypothesis is that if I add the inhibitor (O.5molar Copper Sulphate) to my enzyme (Catalase) and substrate (Hydrogen Peroxide) reaction, the rate of reaction will decrease. Equipment List: O.5 molar solution of Copper Sulphate Hydrogen Peroxide (20 vol) Distilled water 2cm3 of 2.5% yeast solution (which in our case is Catalase) 100cm3 measuring cylinder Water Trough Stopwatch 8 Test tubes Side armed test tube 2cm3 syringe with needle Rubber delivery tube Glass Delivery Tube Half cut bung Clamp This is a diagram of all the equipment listed above. I have chosen this set up of apparatus from a choice of three. I have chosen this apparatus due to faults that could affect the result in the other apparatus. Out of the other two used a manometer. So I immediately eliminated that from the choices available. This is because the dye will be pushed out of the tube, which will make it difficult to record large amounts of oxygen released. The other choice was using the gas syringe. At first I thought the gas syringe would be the most suitable apparatus for this experiment however it had its faults. The tube didn't move very smoothly and this wouldn't give very accurate measurements. So I chose the apparatus in the diagram, on the previous page. This apparatus was well suited to experiment as hardly any oxygen had a chance of escaping Variables controlled: * The amount of enzyme will be kept the same as it will always be kept at 2cm3 * The concentration of enzyme will be kept as accurate as possible as well use the enzyme from the same beaker all throughout the experiment * Temperature will be kept at room ...read more.

Middle

The first test we done, was to carry out the experiment as I have written out before. This was a sort of a trial run to see how the equipment worked and to check if there were any problems. The first test we did gave us no results. This was due to fact that when we collected the yeast from the beaker, we collected the yeast from the top of the beaker. So actually none of the yeast had actually gone into the syringe, only water, as all of the yeast had fallen down to the bottom of the beaker. After this test we decided to stir the yeast before each of the tests. The second test we did, gave us results that we needed. The result is summarized in the table below: Time (seconds) Oxygen Produced (ml) 0 0 10 5 20 10 30 16 40 22 50 28 60 34 70 41 At the next reading the amount of oxygen produced had gone off the scale. This was due to the measuring cylinder, at 50ml, being too small. So we decided after this test to use a 100ml-measuring cylinder instead. Bibliography * 'Biology 1' Jones, M., Fosberry, R. & Taylor, D. Cambridge University Press * www.seps.org * www.bbc.co.uk/revision Results The first table (below) shows the amount of oxygen produced in the reaction without the addition of Copper Sulphate. Time (Seconds) The Amount of Oxygen Produced (cm3) 100% H2O2 75% H2O2 50% H2O2 25% H2O2 0 0 0 0 0 10 13 11 8 6 20 25 22 16 9 30 37 33 24 14 40 51 44 31 18 50 64 54 39 22 60 77 65 46 26 70 89 76 53 30 80 84 59 33 90 65 36 100 71 40 110 78 ...read more.

Conclusion

However, the method did get a bit annoying at times. For example, refilling the measuring cylinder after each experiment. If we used the gas cylinder method it would have been much easier to reset the apparatus after each trial. We did not have any anomalous results as all of the points were near or on the line of best fit. This also meant that the experiment was done accurately. My plan for the method was followed very well. This allowed me to produce very accurate and reliable results. So that means that my method was very well suited to this experiment. The results were also accurate; as I made sure that the meniscus was at eye-level. We did however have limitations in the method. We didn't continue the experiment until the experiment until the reaction had ended. Next time we shouldn't run the experiment for a set amount of time, we should only stop when the reaction itself has ended. The measuring cylinder was too small. Some of the tests made the measuring cylinder fill up with oxygen before the three minutes were up. Next time we should use 200ml or even large measuring cylinder. If possible we could have used more accurate and reliable equipment instead of the measuring cylinder. The unit marks on the measuring cylinder had a difference of 1ml. To increase the accuracy we could have used measuring cylinders that had unit marks every half a ml. Our experiment would have been more reliable if we used a smooth gas syringe. It a piece of equipment specifically designed for collecting gas and would have been very well suited to this experiment. I don't have any sources of error and my experiment was fairly reliable. This means that I have a valid conclusion that, Copper Sulphate is in a fact an inhibitor. Salman Uddin 1 ...read more.

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