• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  6. 6
  7. 7
  8. 8
  9. 9
  10. 10
  11. 11
  12. 12
  13. 13

Find out how Copper Sulphate affects the activity of Catalase.

Extracts from this document...


Enzymes Investigation Aim: To find out how Copper Sulphate affects the activity of Catalase. Hypothesis: My hypothesis is that if I add the inhibitor (O.5molar Copper Sulphate) to my enzyme (Catalase) and substrate (Hydrogen Peroxide) reaction, the rate of reaction will decrease. Equipment List: O.5 molar solution of Copper Sulphate Hydrogen Peroxide (20 vol) Distilled water 2cm3 of 2.5% yeast solution (which in our case is Catalase) 100cm3 measuring cylinder Water Trough Stopwatch 8 Test tubes Side armed test tube 2cm3 syringe with needle Rubber delivery tube Glass Delivery Tube Half cut bung Clamp This is a diagram of all the equipment listed above. I have chosen this set up of apparatus from a choice of three. I have chosen this apparatus due to faults that could affect the result in the other apparatus. Out of the other two used a manometer. So I immediately eliminated that from the choices available. This is because the dye will be pushed out of the tube, which will make it difficult to record large amounts of oxygen released. The other choice was using the gas syringe. At first I thought the gas syringe would be the most suitable apparatus for this experiment however it had its faults. The tube didn't move very smoothly and this wouldn't give very accurate measurements. So I chose the apparatus in the diagram, on the previous page. This apparatus was well suited to experiment as hardly any oxygen had a chance of escaping Variables controlled: * The amount of enzyme will be kept the same as it will always be kept at 2cm3 * The concentration of enzyme will be kept as accurate as possible as well use the enzyme from the same beaker all throughout the experiment * Temperature will be kept at room ...read more.


The first test we done, was to carry out the experiment as I have written out before. This was a sort of a trial run to see how the equipment worked and to check if there were any problems. The first test we did gave us no results. This was due to fact that when we collected the yeast from the beaker, we collected the yeast from the top of the beaker. So actually none of the yeast had actually gone into the syringe, only water, as all of the yeast had fallen down to the bottom of the beaker. After this test we decided to stir the yeast before each of the tests. The second test we did, gave us results that we needed. The result is summarized in the table below: Time (seconds) Oxygen Produced (ml) 0 0 10 5 20 10 30 16 40 22 50 28 60 34 70 41 At the next reading the amount of oxygen produced had gone off the scale. This was due to the measuring cylinder, at 50ml, being too small. So we decided after this test to use a 100ml-measuring cylinder instead. Bibliography * 'Biology 1' Jones, M., Fosberry, R. & Taylor, D. Cambridge University Press * www.seps.org * www.bbc.co.uk/revision Results The first table (below) shows the amount of oxygen produced in the reaction without the addition of Copper Sulphate. Time (Seconds) The Amount of Oxygen Produced (cm3) 100% H2O2 75% H2O2 50% H2O2 25% H2O2 0 0 0 0 0 10 13 11 8 6 20 25 22 16 9 30 37 33 24 14 40 51 44 31 18 50 64 54 39 22 60 77 65 46 26 70 89 76 53 30 80 84 59 33 90 65 36 100 71 40 110 78 ...read more.


However, the method did get a bit annoying at times. For example, refilling the measuring cylinder after each experiment. If we used the gas cylinder method it would have been much easier to reset the apparatus after each trial. We did not have any anomalous results as all of the points were near or on the line of best fit. This also meant that the experiment was done accurately. My plan for the method was followed very well. This allowed me to produce very accurate and reliable results. So that means that my method was very well suited to this experiment. The results were also accurate; as I made sure that the meniscus was at eye-level. We did however have limitations in the method. We didn't continue the experiment until the experiment until the reaction had ended. Next time we shouldn't run the experiment for a set amount of time, we should only stop when the reaction itself has ended. The measuring cylinder was too small. Some of the tests made the measuring cylinder fill up with oxygen before the three minutes were up. Next time we should use 200ml or even large measuring cylinder. If possible we could have used more accurate and reliable equipment instead of the measuring cylinder. The unit marks on the measuring cylinder had a difference of 1ml. To increase the accuracy we could have used measuring cylinders that had unit marks every half a ml. Our experiment would have been more reliable if we used a smooth gas syringe. It a piece of equipment specifically designed for collecting gas and would have been very well suited to this experiment. I don't have any sources of error and my experiment was fairly reliable. This means that I have a valid conclusion that, Copper Sulphate is in a fact an inhibitor. Salman Uddin 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Investigate the effect of copper sulphate on pepsin activity.

    4 star(s)

    Another factor affecting enzyme activity is an inhibitor. An inhibitor is a molecule which can slow down a reaction in two ways, both are illustrated below. Competitive inhibition: Non-competitive inhibition: In competitive inhibition, the inhibitor molecule has a very similar shape to the substrate molecule, so it can fit into the active site of the enzyme.

  2. Marked by a teacher

    effect of concentration of copper sulphate on the action of amylase to break down ...

    4 star(s)

    1 1.0 1.0 4.0 4.0 2.0 Less than a minute Too quick 2 1.0 1.0 1.0 4.0 2.0 About an hour Too slow 3 1.0 1.0 2.0 4.0 2.0 About 2500 Quick 4* 1.0 1.0 2.0 5.0 2.0 About 800 Good timing 5* 0.1 1.0 2.0 5.0 2.0 About 120

  1. Marked by a teacher

    Enzymes - investigate how the substrate concentration (H2O2) affects the activity of catalase on ...

    3 star(s)

    a reliable and accurate set of results that display the effect changing the dependant variable has to this experiment. Below is a table of how these variables will affect the experiment and how it will be controlled. Variable Why is it a variable How it will be controlled Substrate concentration (Independent)

  2. Effects of Copper Sulphate on the Activity of Catalase

    It won't stop it altogether but the affinity will be reduced. Inhibitors like this are often called mixed inhibitors, as they appear to have some of the properties of competitive and non-competitive types. In fact, classical non-competitive inhibitors are very rare, if they exist at all.

  1. Exploring the Effects of Copper Sulphate as an Inhibitor on the Enzyme Amylase

    To dispose of it properly, small amounts of dilute copper sulphate solution can be flushed down a sink with a large quantity of water, unless local rules prohibit this. Larger quantities should be collected as solid waste and disposed of via professional waste disposal.

  2. The aim of this investigation is to find out how concentration of glucose affects ...

    A picture of the structure is shown below 8. ADP + Pi ATP The purpose of this is that ATP has a high energy bond with the last phosphate group a value of 32KJ/mol. This is used to perform other reactions essential to the organisms working. 11. This process of reactions is entirely enzyme controlled and relies on 10 enzymes to work.

  1. Investigate how concentration of the enzyme catalase in celery tissue alters the rate of ...

    This was a good idea as it prevented any loss of oxygen gas which would affect the results and hence my conclusion. Another important and negative point, about the double-holed rubber bungs, and tube which went through it to connect the outside to the inside of the flask, is that

  2. A Level Biology revision notes

    * Occurs in ion uptake by a plant root; glucose uptake by gut cells Ribosomes * 20-30nm in size * Small organelles often attached to the ER but also found in the cytoplasm * Large (protein) and small (rRNA) subunits form the functional ribosome o Subunits bind with mRNA in

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work