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Find the concentration of sucrose in a potato cell - Plan

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Plan For Osmosis/Plasmolysis Experiment AIM In this experiment, I am trying to find the concentration of sucrose in a potato cell. Also I can observe when a cell is totally plasmolyised. PLAN I plan to complete this experiment within an hour. Firstly, I will cut 15 potato chips, 3 each for 5 different solutions, each weighing 1.2grams, cut with a 4mm corer to approx (full word) 4cm. I will measure weight, as length is less accurate. The weight of the chip may vary as much as 0.02 g each way, which will make it a lot easier to do, but may provide me with less accurate results. I will use 5 different molar solutions of 20 cm3 each, shown to the right. 0M (water), 0.5M, 1M, 1.5M and 2M. I will create 0.5 molar solutions by mixing 10cm3 of water and 10cm3 of 1 molar solution. ...read more.


I will use 5 different solutions, starting at 0 molar and going up in 0.5 molar concentrations. I have chosen this because they are equidistant, allowing for easy graph drawing, and also because from the preliminary experiment, I know that this will give me results that can show me the concentration of sucrose in the cells sap, and also go on to show me the concentration that the cell will plasmolyise at. I will also control the variety of potato- I will only use 1 brand, as different brands and types may have different concentrations of sucrose in their cells. . Also all solutions will be measured in beakers to 20 cm3, as if there was a higher volume in one solution, it would take longer to fully even out. My results will be quite reliable if I control the points above, and any anomalies will be identified. ...read more.


These results cannot tell me much, as they do not follow what you would expect. 15 MINS. 1.6g CHIP O MOLAR 1 MOLAR 2 MOLAR CHIP A 1.6g 1.36g 1.31g CHIP B 1.59g 1.41g 1.33g CHIP C 1.59g 1.40g 1.32g AVERAGE 1.593 1.39 1.32 AVERAGE CHANGE -0.44% -13.13% -17.50% Prediction My prediction above is in a slight 's' curve, which is based on the test A of my preliminary. Test A seemed to be the most logical to choose as it followed the patterns that I would expect it to. As we can see from it, the water solution increases its weight, and it shows that the cells concentration is , as this is where it crosses the x-axis. And also it shows us that the cell is fully plasmoliesed at around . this is because the graph starts to level off and that means that the chip is not losing any more weight, because the cell is fully plasmolyised. BY MICHAEL PEARCE ...read more.

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